%0 Journal Article %1 Aviv.1972 %A Aviv, H. %A Leder, P. %D 1972 %J Proc.Natl.Acad.Sci.U.S.A %K & 2 Acrylamides Animals Buffers Carcinoma Cell-Free Centrifugation Chromatography Density Dodecyl Electrophoresis Exchange Globins Gradient Ion Krebs Leucine Messenger Neoplasm Nucleotides Polynucleotides Proteins RNA Rabbits Reticulocytes Ribosomal Sodium Sulfate System Thymine Tritium analysis biosynthesis isolation metabolism methods protein purification %N 6 %P 1408-1412 %T Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose %U PM:4504350 %V 69 %X A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described. The method depends upon annealing poly(adenylic acid)-rich mRNA to oligothymidylic acid-cellulose columns and its elution with buffers of low ionic strength. Biologically active rabbit globin mRNA has been purified by this procedure and assayed for its ability to direct the synthesis of rabbit globin in a cell-free extract of ascites tumor. Inasmuch as various mammalian mRNAs appear to be rich in poly(adenylic acid) and can likely be translated in the ascites cell-free extract, this approach should prove generally useful as an initial step in the isolation of specific mRNAs

@article{Aviv.1972, abstract = {A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described. The method depends upon annealing poly(adenylic acid)-rich mRNA to oligothymidylic acid-cellulose columns and its elution with buffers of low ionic strength. Biologically active rabbit globin mRNA has been purified by this procedure and assayed for its ability to direct the synthesis of rabbit globin in a cell-free extract of ascites tumor. Inasmuch as various mammalian mRNAs appear to be rich in poly(adenylic acid) and can likely be translated in the ascites cell-free extract, this approach should prove generally useful as an initial step in the isolation of specific mRNAs}, added-at = {2010-02-05T11:28:39.000+0100}, author = {Aviv, H. and Leder, P.}, biburl = {https://www.bibsonomy.org/bibtex/2f13c3e5afc1b98d0f85a7b81e81b2941/kanefendt}, interhash = {4fc6182ea5a41cc46b29b1e031e5f327}, intrahash = {f13c3e5afc1b98d0f85a7b81e81b2941}, journal = {Proc.Natl.Acad.Sci.U.S.A}, keywords = {& 2 Acrylamides Animals Buffers Carcinoma Cell-Free Centrifugation Chromatography Density Dodecyl Electrophoresis Exchange Globins Gradient Ion Krebs Leucine Messenger Neoplasm Nucleotides Polynucleotides Proteins RNA Rabbits Reticulocytes Ribosomal Sodium Sulfate System Thymine Tritium analysis biosynthesis isolation metabolism methods protein purification}, number = 6, pages = {1408-1412}, timestamp = {2010-02-05T11:28:44.000+0100}, title = {Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose}, url = {PM:4504350}, volume = 69, year = 1972 }