%0 Generic %1 lorenz2013transforming %A Lorenz, R. %A Moon, E. %A Huang, G. %A Reger, A. %A Kim, J. %A Franz, E. %A Bertinetti, D. %A Kim, C. %A Herberg, F. %D 2013 %J BMC Pharmacology and Toxicology %K herberg ip myown %N Suppl 1 %P P41 %R 10.1186/2050-6511-14-S1-P41 %T Transforming PKA into PKG - a structure-function approach to understand cyclic nucleotide selectivity %U http://www.biomedcentral.com/2050-6511/14/S1/P41 %V 14

@presentation{lorenz2013transforming, added-at = {2013-09-16T15:26:58.000+0200}, author = {Lorenz, R. and Moon, E. and Huang, G. and Reger, A. and Kim, J. and Franz, E. and Bertinetti, D. and Kim, C. and Herberg, F.}, biburl = {https://www.bibsonomy.org/bibtex/21bcfc354859bf0034be1f064ebc3a5d0/biochemie}, description = {cAMP-dependent protein kinase (PKA) and cGMP-dependent protein kinase (PKG) are the main effectors of distinct cyclic nucleotide pathways and are preferentially activated by cAMP or cGMP, respectively. We recently characterized the isolated C-terminal cyclic nucleotide binding domain (CNB-B) of the human PKG Iβ as highly cGMP-selective (manuscript in preparation). In a crystal structure of the CNB-B two novel cGMP-specific interaction sites were identified in addition to the previously described threonine residue (T317) in the phosphate binding cassette [1]. Mutation of each individual site resulted in reduced cGMP-selectivity and interfered with cGMP-dependent activation of PKG Iβ. To gain further insight into the molecular basis of cyclic nucleotide selectivity, we inserted two cGMP-specific interaction sites into the CNB-B of human PKA RIα by mutating corresponding residues. We hypothesize that this way cGMP-specific interaction contacts can be created in PKA and thereby modulate cAMP-selectivity [1,2]. }, doi = {10.1186/2050-6511-14-S1-P41}, interhash = {21c56d41e63c96f6329fc79ac4e1013b}, intrahash = {1bcfc354859bf0034be1f064ebc3a5d0}, issn = {2050-6511}, journal = {BMC Pharmacology and Toxicology}, keywords = {herberg ip myown}, number = {Suppl 1}, pages = {P41}, timestamp = {2016-11-04T11:47:23.000+0100}, title = {Transforming PKA into PKG - a structure-function approach to understand cyclic nucleotide selectivity}, url = {http://www.biomedcentral.com/2050-6511/14/S1/P41}, volume = 14, year = 2013 }