%0 Journal Article %1 barbourNewInsightStimulusinduced2008 %A Barbour, Jon %A Neuhaus, Eva M. %A Piechura, Heike %A Stoepel, Nadine %A Mashukova, Anastasia %A Brunert, Daniela %A Sitek, Barbara %A Stühler, Kai %A Meyer, Helmut E. %A Hatt, Hanns %A Warscheid, Bettina %C United States %D 2008 %J Journal of proteome research %K 2,Calcium-Binding Aldehydes/chemistry,Animals,Calbindin Binding C57BL,Neuronal Calcium G/analysis/metabolism,Smell/physiology,Tandem Gel Inbred Mass Mucosa/*metabolism/physiology,Olfactory Neurons/physiology,Pregnancy,Proteins/analysis/metabolism,Proteomics/*methods,Receptors Odorant/analysis/metabolism,S100 Plasticity/*physiology,Olfactory Protein Proteins/analysis/metabolism,Cytoskeletal Proteins/analysis/metabolism,Down-Regulation,Electrophoresis Psychophysiologic,Lipocalins/analysis/metabolism,Male,Mice,Mice Receptor Spectrometry,to_read,Up-Regulation Two-Dimensional,Electrophysiology,Female,Habituation %N 4 %P 1594--1605 %R 10.1021/pr7005796 %T New Insight into Stimulus-Induced Plasticity of the Olfactory Epithelium in Mus Musculus by Quantitative Proteomics. %V 7 %X The olfactory system is exposed to a plethora of chemical compounds throughout an organism's lifespan. Anticipation of stimuli and construction of appropriate neural filters present a significant challenge. This may be addressed via modulation of the protein composition of the sensory epithelium in response to environmental conditions. To reveal the mechanisms governing these changes, we employed a comprehensive quantitative proteomics strategy. Two groups of juvenile mice were treated with either pulsed or continuous application of octanal. After 20 days of treatment, we performed a behavioral study and conducted electrophysiological recordings from the olfactory epithelium (OE). Both treated groups demonstrated peripheral desensitization to octanal; however, only the 'continuous' group exhibited habituation. To obtain novel insight into the molecular mechanisms underpinning the peripheral desensitization to octanal, the OE proteomes of octanal-treated mice versus control were quantitatively analyzed using two-dimensional difference gel electrophoresis. We identified several significantly regulated proteins that were functionally classified as calcium-binding proteins, cytoskeletal proteins, and lipocalins. The calcium-binding proteins and cytoskeletal proteins were up-regulated in the 'pulsed' group, whereas in the 'continuous' group, four lipocalins were significantly down-regulated. Uniquely, the lipocalin odorant-binding protein Ia was drastically down-regulated in both groups. The identified proteins reflect changes throughout the entire OE, corresponding to changes in neuronal, non-neuronal, and pericellular processes. We report the regulation of several promising candidates for the investigation of odorant-induced changes of the OE. Among these proteins are different lipocalins, which seem to play a crucial role in the regulation of the sensitivity of the olfactory system.

@article{barbourNewInsightStimulusinduced2008, abstract = {The olfactory system is exposed to a plethora of chemical compounds throughout an organism's lifespan. Anticipation of stimuli and construction of appropriate neural filters present a significant challenge. This may be addressed via modulation of the protein composition of the sensory epithelium in response to environmental conditions. To reveal the mechanisms governing these changes, we employed a comprehensive quantitative proteomics strategy. Two groups of juvenile mice were treated with either pulsed or continuous application of octanal. After 20 days of treatment, we performed a behavioral study and conducted electrophysiological recordings from the olfactory epithelium (OE). Both treated groups demonstrated peripheral desensitization to octanal; however, only the 'continuous' group exhibited habituation. To obtain novel insight into the molecular mechanisms underpinning the peripheral desensitization to octanal, the OE proteomes of octanal-treated mice versus control were quantitatively analyzed using two-dimensional difference gel electrophoresis. We identified several significantly regulated proteins that were functionally classified as calcium-binding proteins, cytoskeletal proteins, and lipocalins. The calcium-binding proteins and cytoskeletal proteins were up-regulated in the 'pulsed' group, whereas in the 'continuous' group, four lipocalins were significantly down-regulated. Uniquely, the lipocalin odorant-binding protein Ia was drastically down-regulated in both groups. The identified proteins reflect changes throughout the entire OE, corresponding to changes in neuronal, non-neuronal, and pericellular processes. We report the regulation of several promising candidates for the investigation of odorant-induced changes of the OE. Among these proteins are different lipocalins, which seem to play a crucial role in the regulation of the sensitivity of the olfactory system.}, added-at = {2024-05-17T13:01:35.000+0200}, address = {United States}, author = {Barbour, Jon and Neuhaus, Eva M. and Piechura, Heike and Stoepel, Nadine and Mashukova, Anastasia and Brunert, Daniela and Sitek, Barbara and St{\"u}hler, Kai and Meyer, Helmut E. and Hatt, Hanns and Warscheid, Bettina}, biburl = {https://www.bibsonomy.org/bibtex/28850ed4e7efa6481ea156191bb60d27f/warscheidlab}, doi = {10.1021/pr7005796}, interhash = {2c6058607d5d2a5df1d8ce54b2f7e93d}, intrahash = {8850ed4e7efa6481ea156191bb60d27f}, issn = {1535-3893}, journal = {Journal of proteome research}, keywords = {2,Calcium-Binding Aldehydes/chemistry,Animals,Calbindin Binding C57BL,Neuronal Calcium G/analysis/metabolism,Smell/physiology,Tandem Gel Inbred Mass Mucosa/*metabolism/physiology,Olfactory Neurons/physiology,Pregnancy,Proteins/analysis/metabolism,Proteomics/*methods,Receptors Odorant/analysis/metabolism,S100 Plasticity/*physiology,Olfactory Protein Proteins/analysis/metabolism,Cytoskeletal Proteins/analysis/metabolism,Down-Regulation,Electrophoresis Psychophysiologic,Lipocalins/analysis/metabolism,Male,Mice,Mice Receptor Spectrometry,to_read,Up-Regulation Two-Dimensional,Electrophysiology,Female,Habituation}, langid = {english}, month = apr, number = 4, pages = {1594--1605}, pmid = {18336002}, timestamp = {2024-05-17T13:01:35.000+0200}, title = {New Insight into Stimulus-Induced Plasticity of the Olfactory Epithelium in {{Mus}} Musculus by Quantitative Proteomics.}, volume = 7, year = 2008 }