Abstract
Application of single-molecule switching nanoscopy (SMSN) beyond the coverslip surface poses substantial challenges due to sample-induced aberrations that distort and blur single-molecule emission patterns. We combined active shaping of point spread functions and efficient adaptive optics to enable robust 3D-SMSN imaging within tissues. This development allowed us to image through 30-$\mu$m-thick brain sections to visualize and reconstruct the morphology and the nanoscale details of amyloid-$\beta$ filaments in a mouse model of Alzheimer's disease.
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