%0 Journal Article %1 yi2004small %A Yi, L %A Li, Z %A Yuan, K %A Qu, X %A Chen, J %A Wang, G %A Zhang, H %A Luo, H %A Zhu, L %A Jiang, P %A Chen, L %A Shen, Y %A Luo, M %A Zuo, G %A Hu, J %A Duan, D %A Nie, Y %A Shi, X %A Wang, W %A Han, Y %A Li, T %A Liu, Y %A Ding, M %A Deng, H %A Xu, X %D 2004 %J J Virol %K SARS_1 quercetin %N 20 %P 11334-11339 %R 10.1128/JVI.78.20.11334-11339.2004 %T Small molecules blocking the entry of severe acute respiratory syndrome coronavirus into host cells %U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC521800/ %V 78 %X Severe acute respiratory syndrome coronavirus (SARS-CoV) is the pathogen of SARS, which caused a global panic in 2003. We describe here the screening of Chinese herbal medicine-based, novel small molecules that bind avidly with the surface spike protein of SARS-CoV and thus can interfere with the entry of the virus to its host cells. We achieved this by using a two-step screening method consisting of frontal affinity chromatography-mass spectrometry coupled with a viral infection assay based on a human immunodeficiency virus (HIV)-luc/SARS pseudotyped virus. Two small molecules, tetra-O-galloyl-beta-D-glucose (TGG) and luteolin, were identified, whose anti-SARS-CoV activities were confirmed by using a wild-type SARS-CoV infection system. TGG exhibits prominent anti-SARS-CoV activity with a 50% effective concentration of 4.5 microM and a selective index of 240.0. The two-step screening method described here yielded several small molecules that can be used for developing new classes of anti-SARS-CoV drugs and is potentially useful for the high-throughput screening of drugs inhibiting the entry of HIV, hepatitis C virus, and other insidious viruses into their host cells.

@article{yi2004small, abstract = {Severe acute respiratory syndrome coronavirus (SARS-CoV) is the pathogen of SARS, which caused a global panic in 2003. We describe here the screening of Chinese herbal medicine-based, novel small molecules that bind avidly with the surface spike protein of SARS-CoV and thus can interfere with the entry of the virus to its host cells. We achieved this by using a two-step screening method consisting of frontal affinity chromatography-mass spectrometry coupled with a viral infection assay based on a human immunodeficiency virus (HIV)-luc/SARS pseudotyped virus. Two small molecules, tetra-O-galloyl-beta-D-glucose (TGG) and luteolin, were identified, whose anti-SARS-CoV activities were confirmed by using a wild-type SARS-CoV infection system. TGG exhibits prominent anti-SARS-CoV activity with a 50% effective concentration of 4.5 microM and a selective index of 240.0. The two-step screening method described here yielded several small molecules that can be used for developing new classes of anti-SARS-CoV drugs and is potentially useful for the high-throughput screening of drugs inhibiting the entry of HIV, hepatitis C virus, and other insidious viruses into their host cells.}, added-at = {2020-07-12T16:49:25.000+0200}, author = {Yi, L and Li, Z and Yuan, K and Qu, X and Chen, J and Wang, G and Zhang, H and Luo, H and Zhu, L and Jiang, P and Chen, L and Shen, Y and Luo, M and Zuo, G and Hu, J and Duan, D and Nie, Y and Shi, X and Wang, W and Han, Y and Li, T and Liu, Y and Ding, M and Deng, H and Xu, X}, biburl = {https://www.bibsonomy.org/bibtex/2ec3ada58652c09c5d59d3c24de988b99/fordham1}, description = {Small Molecules Blocking the Entry of Severe Acute Respiratory Syndrome Coronavirus into Host Cells}, doi = {10.1128/JVI.78.20.11334-11339.2004}, interhash = {09883859a6847b5e069c8570aec68a8f}, intrahash = {ec3ada58652c09c5d59d3c24de988b99}, journal = {J Virol}, keywords = {SARS_1 quercetin}, month = oct, number = 20, pages = {11334-11339}, pmid = {15452254}, timestamp = {2020-07-12T16:49:25.000+0200}, title = {Small molecules blocking the entry of severe acute respiratory syndrome coronavirus into host cells}, url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC521800/}, volume = 78, year = 2004 }