%0 Journal Article %1 Schueder2019 %A Schueder, Florian %A Stein, Johannes %A Stehr, Florian %A Auer, Alexander %A Sperl, Bianca %A Strauss, Maximilian T. %A Schwille, Petra %A Jungmann, Ralf %D 2019 %J Nature Methods %K dna-paint imaging microscopy speedpaint superresolution %R 10.1038/s41592-019-0584-7 %T An order of magnitude faster DNA-PAINT imaging by optimized sequence design and buffer conditions %X DNA points accumulation in nanoscale topography (DNA-PAINT) is a relatively easy-to-implement super-resolution technique. However, image acquisition is slow compared to most other approaches. Here, we overcome this limitation by designing optimized DNA sequences and buffer conditions. We demonstrate our approach in vitro with DNA origami and in situ using cell samples, and achieve an order of magnitude faster imaging speeds without compromising image quality or spatial resolution. This improvement now makes DNA-PAINT applicable to high-throughput studies. DNA-PAINT is sped up by an order of magnitude by optimizing sequences and buffer conditions, enabling faster imaging with no compromise to image quality or resolution, improved single-molecule counting and enhanced cellular imaging.

@article{Schueder2019, abstract = {DNA points accumulation in nanoscale topography (DNA-PAINT) is a relatively easy-to-implement super-resolution technique. However, image acquisition is slow compared to most other approaches. Here, we overcome this limitation by designing optimized DNA sequences and buffer conditions. We demonstrate our approach in vitro with DNA origami and in situ using cell samples, and achieve an order of magnitude faster imaging speeds without compromising image quality or spatial resolution. This improvement now makes DNA-PAINT applicable to high-throughput studies. DNA-PAINT is sped up by an order of magnitude by optimizing sequences and buffer conditions, enabling faster imaging with no compromise to image quality or resolution, improved single-molecule counting and enhanced cellular imaging.}, added-at = {2020-03-23T21:12:34.000+0100}, author = {Schueder, Florian and Stein, Johannes and Stehr, Florian and Auer, Alexander and Sperl, Bianca and Strauss, Maximilian T. and Schwille, Petra and Jungmann, Ralf}, biburl = {https://www.bibsonomy.org/bibtex/2edd2c80d6948d84ba84b0f592ec88591/kfriedl}, doi = {10.1038/s41592-019-0584-7}, interhash = {262bce42e2070a0e964ffbab07b343d6}, intrahash = {edd2c80d6948d84ba84b0f592ec88591}, issn = {1548-7091}, journal = {Nature Methods}, keywords = {dna-paint imaging microscopy speedpaint superresolution}, timestamp = {2020-03-23T22:13:28.000+0100}, title = {{An order of magnitude faster DNA-PAINT imaging by optimized sequence design and buffer conditions}}, year = 2019 }