%0 Journal Article %1 citeulike:488454 %A Sugawara, Kazuharu %A Hirabayashi, George %A Kamiya, Naoto %A Kuramitz, Hideki %A Tanaka, Shunitz %C Kazuharu Sugawara, 1Faculty of Education, Gunma University, Maebashi, Gunma, 371-8510 Japan %D 2005 %J Electroanalysis %K labeled_mediator competition homogeneous ag_detection electrochemistry %N 18 %P 1659--1664 %R 10.1002/elan.200503265 %T Evaluation of Binding Between Electroactive Biotin Derivative and Streptavidin Immobilized on Chitin Film %U http://dx.doi.org/10.1002/elan.200503265 %V 17 %X Evaluation of the streptavidin-biotin binding at the surface of chitin film was carried out with voltammetry. Immobilization of streptavidin was attempted to the protonated chitin film, based on an electrostatic interaction that hardly causes any change in the protein structure. The streptavidin-biotin binding was estimated from changes in the electrode response of biotin labeled with an electroactive compound. Although the response of daunomycin as an electroactive compound did not change at an electrode covered with streptavidin/chitin film, the response of the labeled biotin decreased. This observation shows that streptavidin is immobilized on the chitin film and the biotin binds with immobilized streptavidin. Consequently, it was clear that the chitin film is useful as a reaction field for protein-ligand binding. Generally, a binding event between protein and its ligand in the living body occurs on the cell surface. The electrochemical evaluation of protein-ligand binding on a natural polysaccharide like chitin membrane surface is important.

@article{citeulike:488454, abstract = {Evaluation of the streptavidin-biotin binding at the surface of chitin film was carried out with voltammetry. Immobilization of streptavidin was attempted to the protonated chitin film, based on an electrostatic interaction that hardly causes any change in the protein structure. The streptavidin-biotin binding was estimated from changes in the electrode response of biotin labeled with an electroactive compound. Although the response of daunomycin as an electroactive compound did not change at an electrode covered with streptavidin/chitin film, the response of the labeled biotin decreased. This observation shows that streptavidin is immobilized on the chitin film and the biotin binds with immobilized streptavidin. Consequently, it was clear that the chitin film is useful as a reaction field for protein-ligand binding. Generally, a binding event between protein and its ligand in the living body occurs on the cell surface. The electrochemical evaluation of protein-ligand binding on a natural polysaccharide like chitin membrane surface is important.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Kazuharu Sugawara, 1Faculty of Education, Gunma University, Maebashi, Gunma, 371-8510 Japan}, author = {Sugawara, Kazuharu and Hirabayashi, George and Kamiya, Naoto and Kuramitz, Hideki and Tanaka, Shunitz}, biburl = {https://www.bibsonomy.org/bibtex/2fec4510c72f426df283948d72731db08/biblio24}, citeulike-article-id = {488454}, comment = {kzsuga@edu.gunma-u.ac.jp}, doi = {10.1002/elan.200503265}, interhash = {2e06fada6ec0f39e2c83206c49762b22}, intrahash = {fec4510c72f426df283948d72731db08}, journal = {Electroanalysis}, keywords = {labeled_mediator competition homogeneous ag_detection electrochemistry}, month = {September}, number = 18, pages = {1659--1664}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Evaluation of Binding Between Electroactive Biotin Derivative and Streptavidin Immobilized on Chitin Film}, url = {http://dx.doi.org/10.1002/elan.200503265}, volume = 17, year = 2005 }