%0 Journal Article %1 RN240 %A Gil-Duran, C. %A Rojas-Aedo, J.F. %A Medina, E. %A Vaca, I. %A Garcia-Rico, R.O. %A Villagran, S. %A Levican, G. %A Chavez, R. %D 2015 %J Plos One %K alpha-subunit, aspergillus-nidulans, chrysogenum, cluster, dqcauchile pga1 %N 3 %R 10.1371/journal.pone.0120740 %T The Pcz1 Gene, Which Encodes a Zn(Ii)(2)Cys(6) Protein, Is Involved in the Control of Growth, Conidiation, and Conidial Germination in the Filamentous Fungus Penicillium Roqueforti %U /brokenurl#<Go to ISI>://WOS:000356353700059 %V 10 %X Proteins containing Zn(II)(2)Cys(6) domains are exclusively found in fungi and yeasts. Genes encoding this class of proteins are broadly distributed in fungi, but few of them have been functionally characterized. In this work, we have characterized a gene from the filamentous fungus Penicillium roqueforti that encodes a Zn(II)(2)Cys(6) protein, whose function to date remains unknown. We have named this gene pcz1. We showed that the expression of pcz1 is negatively regulated in a P. roqueforti strain containing a dominant active Gai protein, suggesting that pcz1 encodes a downstream effector that is negatively controlled by Gai. More interestingly, the silencing of pcz1 in P. roqueforti using RNAi-silencing technology resulted in decreased apical growth, the promotion of conidial germination (even in the absence of a carbon source), and the strong repression of conidiation, concomitant with the downregulation of the genes of the central conidiation pathway brlA, abaA and wetA. A model for the participation of pcz1 in these physiological processes in P. roqueforti is proposed.

@article{RN240, abstract = {Proteins containing Zn(II)(2)Cys(6) domains are exclusively found in fungi and yeasts. Genes encoding this class of proteins are broadly distributed in fungi, but few of them have been functionally characterized. In this work, we have characterized a gene from the filamentous fungus Penicillium roqueforti that encodes a Zn(II)(2)Cys(6) protein, whose function to date remains unknown. We have named this gene pcz1. We showed that the expression of pcz1 is negatively regulated in a P. roqueforti strain containing a dominant active Gai protein, suggesting that pcz1 encodes a downstream effector that is negatively controlled by Gai. More interestingly, the silencing of pcz1 in P. roqueforti using RNAi-silencing technology resulted in decreased apical growth, the promotion of conidial germination (even in the absence of a carbon source), and the strong repression of conidiation, concomitant with the downregulation of the genes of the central conidiation pathway brlA, abaA and wetA. A model for the participation of pcz1 in these physiological processes in P. roqueforti is proposed.}, added-at = {2019-12-04T03:57:35.000+0100}, author = {Gil-Duran, C. and Rojas-Aedo, J.F. and Medina, E. and Vaca, I. and Garcia-Rico, R.O. and Villagran, S. and Levican, G. and Chavez, R.}, biburl = {https://www.bibsonomy.org/bibtex/224a4919e9b53a371c6fa6278a34b374e/dqcauchile}, doi = {10.1371/journal.pone.0120740}, interhash = {362dd1fa561b6f925c7eb22ca115abe4}, intrahash = {24a4919e9b53a371c6fa6278a34b374e}, issn = {1932-6203}, journal = {Plos One}, keywords = {alpha-subunit, aspergillus-nidulans, chrysogenum, cluster, dqcauchile pga1}, number = 3, timestamp = {2019-12-04T03:58:17.000+0100}, title = {The Pcz1 Gene, Which Encodes a Zn(Ii)(2)Cys(6) Protein, Is Involved in the Control of Growth, Conidiation, and Conidial Germination in the Filamentous Fungus Penicillium Roqueforti}, type = {Journal Article}, url = {/brokenurl#<Go to ISI>://WOS:000356353700059}, volume = 10, year = 2015 }