%0 Journal Article %1 citeulike:477563 %A Hock, B. %A Rahman, M. %A Rauchalles, S. %A Dankwardt, A. %A Seifert, M. %A Haindl, S. %A Kramer, K. %D 1999 %J Journal of Molecular Catalysis B: Enzymatic %K methodology immunoassay antibody %N 1-4 %P 115--124 %R 10.1016/S1381-1177(99)00036-3 %T Stabilisation of immunoassays and receptor assays %U http://www.sciencedirect.com/science/article/B6TGN-3X9JG57-F/2/e17e29ac4886bbcfccea1fd243151ed7 %V 7 %X Stability of immunoassays and receptor assays depends on the integrity of antibodies and receptors as binding proteins for the analytes. In addition, coating conjugates, tracers, and if applicable, enzyme substrates have to be considered. In this study, temperature effects on monoclonal and recombinant antibodies for atrazine and on estrogen receptors were investigated in the presence and absence of stabilisers. Monoclonal antibodies for atrazine suffered only moderate activity losses after a 10-day incubation at 37degC which could be demonstrated by shifts of the calibration curves. Even more remarkable was the resistance of the analogous recombinant Fab fragments to temperatures up to 50degC. However, the estrogen receptor showed severe losses already after a 3-day exposure at 4degC. Various stabilisers were tested. In the case of antibodies, several commercial stabilisers prevented activity losses at higher temperatures. Improved stabilisation strategies are discussed. They are mainly seen in the selection of temperature-resistant recombinant antibodies and in the reduction of assay components.

@article{citeulike:477563, abstract = {Stability of immunoassays and receptor assays depends on the integrity of antibodies and receptors as binding proteins for the analytes. In addition, coating conjugates, tracers, and if applicable, enzyme substrates have to be considered. In this study, temperature effects on monoclonal and recombinant antibodies for atrazine and on estrogen receptors were investigated in the presence and absence of stabilisers. Monoclonal antibodies for atrazine suffered only moderate activity losses after a 10-day incubation at 37[deg]C which could be demonstrated by shifts of the calibration curves. Even more remarkable was the resistance of the analogous recombinant Fab fragments to temperatures up to 50[deg]C. However, the estrogen receptor showed severe losses already after a 3-day exposure at 4[deg]C. Various stabilisers were tested. In the case of antibodies, several commercial stabilisers prevented activity losses at higher temperatures. Improved stabilisation strategies are discussed. They are mainly seen in the selection of temperature-resistant recombinant antibodies and in the reduction of assay components.}, added-at = {2006-07-07T01:10:50.000+0200}, author = {Hock, B. and Rahman, M. and Rauchalles, S. and Dankwardt, A. and Seifert, M. and Haindl, S. and Kramer, K.}, biburl = {https://www.bibsonomy.org/bibtex/2a30aca522996ede82f1c0509827e6c06/biblio24}, citeulike-article-id = {477563}, doi = {10.1016/S1381-1177(99)00036-3}, interhash = {18a96a5f3ff1a328560d0b2897aaffe7}, intrahash = {a30aca522996ede82f1c0509827e6c06}, journal = {Journal of Molecular Catalysis B: Enzymatic}, keywords = {methodology immunoassay antibody}, month = {September}, number = {1-4}, pages = {115--124}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Stabilisation of immunoassays and receptor assays}, url = {http://www.sciencedirect.com/science/article/B6TGN-3X9JG57-F/2/e17e29ac4886bbcfccea1fd243151ed7}, volume = 7, year = 1999 }