%0 Journal Article %1 RN1206 %A Szyroki, A. %A Ivashikina, N. %A Dietrich, P. %A Roelfsema, M. R. G. %A Ache, P. %A Reintanz, B. %A Deeken, R. %A Godde, M. %A Felle, H. %A Steinmeyer, R. %A Palme, K. %A Hedrich, R. %D 2001 %J Proceedings of the National Academy of Sciences of the United States of America %K myOwn plant plasma-membrane %N 5 %P 2917-2921 %R DOI 10.1073/pnas.051616698 %T KAT1 is not essential for stomatal opening %U /brokenurl#<Go to ISI>://WOS:000167258900145 %V 98 %X It is generally accepted that Kf uptake into guard cells via inward-rectifying K+ channels is required for stomatal opening. To test whether the guard cell K+ channel KAT1 is essential for stomatal opening, a knockout mutant, KAT1::En-1, was isolated from an En-l mutagenized Arabidopsis thaliana population. Stomatal action and K+ uptake, however, were not impaired in KAT1-deficient plants. Reverse transcription-PCR experiments with isolated guard cell protoplasts showed that in addition to KAT1, the Kf channels AKT1, AKT2/3, AtKC1, and KAT2 were expressed in this cell type. In impalement measurements, intact guard cells exhibited inward-rectifying Kf currents across the plasma membrane of both wildtype and KAT1::En-1 plants. This study demonstrates that multiple K+ channel transcripts exist in guard cells and that KAT1 is not essential for stomatal action.

@article{RN1206, abstract = {It is generally accepted that Kf uptake into guard cells via inward-rectifying K+ channels is required for stomatal opening. To test whether the guard cell K+ channel KAT1 is essential for stomatal opening, a knockout mutant, KAT1::En-1, was isolated from an En-l mutagenized Arabidopsis thaliana population. Stomatal action and K+ uptake, however, were not impaired in KAT1-deficient plants. Reverse transcription-PCR experiments with isolated guard cell protoplasts showed that in addition to KAT1, the Kf channels AKT1, AKT2/3, AtKC1, and KAT2 were expressed in this cell type. In impalement measurements, intact guard cells exhibited inward-rectifying Kf currents across the plasma membrane of both wildtype and KAT1::En-1 plants. This study demonstrates that multiple K+ channel transcripts exist in guard cells and that KAT1 is not essential for stomatal action.}, added-at = {2024-02-14T14:38:32.000+0100}, author = {Szyroki, A. and Ivashikina, N. and Dietrich, P. and Roelfsema, M. R. G. and Ache, P. and Reintanz, B. and Deeken, R. and Godde, M. and Felle, H. and Steinmeyer, R. and Palme, K. and Hedrich, R.}, biburl = {https://www.bibsonomy.org/bibtex/2410b19cec1669b89b23b6c77341e3823/rainerhedrich_2}, doi = {DOI 10.1073/pnas.051616698}, interhash = {c70d7874991b2fe528e07960eaf84ef7}, intrahash = {410b19cec1669b89b23b6c77341e3823}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {myOwn plant plasma-membrane}, note = {407eh Times Cited:182 Cited References Count:47}, number = 5, pages = {2917-2921}, timestamp = {2024-02-14T14:38:32.000+0100}, title = {KAT1 is not essential for stomatal opening}, type = {Journal Article}, url = {/brokenurl#<Go to ISI>://WOS:000167258900145}, volume = 98, year = 2001 }