%0 Journal Article %1 wang2009rnaseq %A Wang, Zhong %A Gerstein, Mark %A Snyder, Michael %D 2009 %J Nat Rev Genet %K dna methods sequencing %N 1 %P 57-63 %R 10.1038/nrg2484 %T RNA-Seq: a revolutionary tool for transcriptomics %U https://www.ncbi.nlm.nih.gov/pubmed/19015660 %V 10 %X RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.

@article{wang2009rnaseq, abstract = {RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.}, added-at = {2017-12-03T20:49:06.000+0100}, author = {Wang, Zhong and Gerstein, Mark and Snyder, Michael}, biburl = {https://www.bibsonomy.org/bibtex/2e2fd562e87a2303d12baa48e971f1e2a/artheibault}, description = {This article describes RNA-seq, a technique used to sequence cDNA directly from mRNA transcripts. At the time, it improved upon some of the limitations of microarrays, and many studies continue to use it to determine transcription levels of genes.}, doi = {10.1038/nrg2484}, interhash = {d4e6fc1efc7df7048bea18359c00a593}, intrahash = {e2fd562e87a2303d12baa48e971f1e2a}, journal = {Nat Rev Genet}, keywords = {dna methods sequencing}, number = 1, pages = {57-63}, timestamp = {2017-12-03T20:49:06.000+0100}, title = {RNA-Seq: a revolutionary tool for transcriptomics}, url = {https://www.ncbi.nlm.nih.gov/pubmed/19015660}, volume = 10, year = 2009 }