%0 Journal Article %1 amatobi2023circadian %A Amatobi, K. M. %A Ozbek-Unal, A. G. %A Schabler, S. %A Deppisch, P. %A Helfrich-Forster, C. %A Mueller, M. J. %A Wegener, C. %A Fekete, A. %D 2023 %J J Lipid Res %K circadian myOwn rhythm uni_network %N 10 %P 100417 %R 10.1016/j.jlr.2023.100417 %T The circadian clock is required for rhythmic lipid transport in Drosophila in interaction with diet and photic condition %U https://www.ncbi.nlm.nih.gov/pubmed/37481037https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10550813/pdf/main.pdf %V 64 %X Modern lifestyle is often at odds with endogenously driven rhythmicity, which can lead to circadian disruption and metabolic syndrome. One signature for circadian disruption is a reduced or altered metabolite cycling in the circulating tissue reflecting the current metabolic status. Drosophila is a well-established model in chronobiology, but day-time dependent variations of transport metabolites in the fly circulation are poorly characterized. Here, we sampled fly hemolymph throughout the day and analyzed diacylglycerols (DGs), phosphoethanolamines (PEs) and phosphocholines (PCs) using LC-MS. In wild-type flies kept on sugar-only medium under a light-dark cycle, all transport lipid species showed a synchronized bimodal oscillation pattern with maxima at the beginning and end of the light phase which were impaired in period(01) clock mutants. In wild-type flies under constant dark conditions, the oscillation became monophasic with a maximum in the middle of the subjective day. In strong support of clock-driven oscillations, levels of the targeted lipids peaked once in the middle of the light phase under time-restricted feeding independent of the time of food intake. When wild-type flies were reared on full standard medium, the rhythmic alterations of hemolymph lipid levels were greatly attenuated. Our data suggest that the circadian clock aligns daily oscillations of DGs, PEs, and PCs in the hemolymph to the anabolic siesta phase, with a strong influence of light on phase and modality.

@article{amatobi2023circadian, abstract = {Modern lifestyle is often at odds with endogenously driven rhythmicity, which can lead to circadian disruption and metabolic syndrome. One signature for circadian disruption is a reduced or altered metabolite cycling in the circulating tissue reflecting the current metabolic status. Drosophila is a well-established model in chronobiology, but day-time dependent variations of transport metabolites in the fly circulation are poorly characterized. Here, we sampled fly hemolymph throughout the day and analyzed diacylglycerols (DGs), phosphoethanolamines (PEs) and phosphocholines (PCs) using LC-MS. In wild-type flies kept on sugar-only medium under a light-dark cycle, all transport lipid species showed a synchronized bimodal oscillation pattern with maxima at the beginning and end of the light phase which were impaired in period(01) clock mutants. In wild-type flies under constant dark conditions, the oscillation became monophasic with a maximum in the middle of the subjective day. In strong support of clock-driven oscillations, levels of the targeted lipids peaked once in the middle of the light phase under time-restricted feeding independent of the time of food intake. When wild-type flies were reared on full standard medium, the rhythmic alterations of hemolymph lipid levels were greatly attenuated. Our data suggest that the circadian clock aligns daily oscillations of DGs, PEs, and PCs in the hemolymph to the anabolic siesta phase, with a strong influence of light on phase and modality.}, added-at = {2024-02-15T15:08:22.000+0100}, author = {Amatobi, K. M. and Ozbek-Unal, A. G. and Schabler, S. and Deppisch, P. and Helfrich-Forster, C. and Mueller, M. J. and Wegener, C. and Fekete, A.}, biburl = {https://www.bibsonomy.org/bibtex/2e67e07737c60f7ef32b294a7382b3bdc/jvsi_all}, doi = {10.1016/j.jlr.2023.100417}, interhash = {e9e9aecdb27cc229cb86d03daee858e4}, intrahash = {e67e07737c60f7ef32b294a7382b3bdc}, issn = {1539-7262 (Electronic) 0022-2275 (Print) 0022-2275 (Linking)}, journal = {J Lipid Res}, keywords = {circadian myOwn rhythm uni_network}, note = {Amatobi, Kelechi M Ozbek-Unal, Ayten Gizem Schabler, Stefan Deppisch, Peter Helfrich-Forster, Charlotte Mueller, Martin J Wegener, Christian Fekete, Agnes eng 2023/07/23 J Lipid Res. 2023 Oct;64(10):100417. doi: 10.1016/j.jlr.2023.100417. Epub 2023 Jul 20.}, number = 10, pages = 100417, timestamp = {2024-02-15T15:11:55.000+0100}, title = {The circadian clock is required for rhythmic lipid transport in Drosophila in interaction with diet and photic condition}, type = {Journal Article}, url = {https://www.ncbi.nlm.nih.gov/pubmed/37481037https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10550813/pdf/main.pdf}, volume = 64, year = 2023 }