%0 Journal Article %1 WOS:000537562100034 %A Cruz, Beatriz G %A dos Santos, Helcio S %A Bandeira, Paulo N %A Rodrigues, Tigressa Helena S %A Matos, Maria Geysillene C %A Nascimento, Mariana F %A de Carvalho, Guilherme G C %A Braz-Filho, Raimundo %A Teixeira, Alexandre M R %A Tintino, Saulo R %A Coutinho, Henrique D M %C 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND %D 2020 %I ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD %J MICROBIAL PATHOGENESIS %K Antibacterial Multidrug-resistant activity; bacteria} kaempferol; {Flavonoid %R 10.1016/j.micpath.2020.104144 %T Evaluation of antibacterial and enhancement of antibiotic action by the flavonoid kaempferol 7-O-beta-D-(6 `'-O-cumaroyl)-glucopyranoside isolated from Croton piauhiensis mull %V 143 %X There has been a rapid increase in the incidence and prevalence of opportunistic bacterial infections. Inappropriate use of current antibiotics has continuously contributed to the emergence of resistance to conventional antibiotic therapy. Therefore, the search for natural molecules that are able to combat infections is of great public interest, and many of these compounds with antimicrobial properties can be obtained from phytochemical studies of medicinal plants. In this context, this study reports the isolation and characterization of the flavonoid, kaempferol 7-O-beta-D-(6-''-O-cumaroyl)-glucopyranoside, from Croton piauhiensis leaves. Additionally, the intrinsic antimicrobial action of the compound and its enhancement against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus strains were assessed. The minimum inhibitory concentration (MIC) of the compound was determined using broth microdilution assays. To evaluate the modulatory effect of the flavonoid, the MIC of antibiotics amikacin and gentamicin, belonging to the class aminoglycosides was assessed, with and without the compound in sterile microplates. The results of intrinsic antibacterial activity tests revealed that the compound had no antibacterial activity against strains tested at concentrations < 1024 mu g/mL. The combination of the flavonoid at a concentration of 128 mu g/mL with gentamicin presented synergistic effects against S. aureus 10 and E. coli 06, and also reduced the MIC from 16 mu g/mL to 4 mu g/mL and 8 mu g/mL, respectively. Amikacin also showed synergistic effects against S. aureus 10 and E. coli 06. We also observed reduced MIC for both, from 128 mu g/mL to 32 mu g/mL; however, antagonism for P. aeruginosa increased the MIC from 16 mu g/mL to 64 mu g/mL. The combination of the flavonoid with the aminoglycosides may be an alternative to potentiate the expected results in treatment against S. aureus and E. coli, since their association leads to a synergistic effect, reducing the MIC of these drugs and decreasing the dose necessary for therapeutic success.

@article{WOS:000537562100034, abstract = {There has been a rapid increase in the incidence and prevalence of opportunistic bacterial infections. Inappropriate use of current antibiotics has continuously contributed to the emergence of resistance to conventional antibiotic therapy. Therefore, the search for natural molecules that are able to combat infections is of great public interest, and many of these compounds with antimicrobial properties can be obtained from phytochemical studies of medicinal plants. In this context, this study reports the isolation and characterization of the flavonoid, kaempferol 7-O-beta-D-(6-''-O-cumaroyl)-glucopyranoside, from Croton piauhiensis leaves. Additionally, the intrinsic antimicrobial action of the compound and its enhancement against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus strains were assessed. The minimum inhibitory concentration (MIC) of the compound was determined using broth microdilution assays. To evaluate the modulatory effect of the flavonoid, the MIC of antibiotics amikacin and gentamicin, belonging to the class aminoglycosides was assessed, with and without the compound in sterile microplates. The results of intrinsic antibacterial activity tests revealed that the compound had no antibacterial activity against strains tested at concentrations < 1024 mu g/mL. The combination of the flavonoid at a concentration of 128 mu g/mL with gentamicin presented synergistic effects against S. aureus 10 and E. coli 06, and also reduced the MIC from 16 mu g/mL to 4 mu g/mL and 8 mu g/mL, respectively. Amikacin also showed synergistic effects against S. aureus 10 and E. coli 06. We also observed reduced MIC for both, from 128 mu g/mL to 32 mu g/mL; however, antagonism for P. aeruginosa increased the MIC from 16 mu g/mL to 64 mu g/mL. The combination of the flavonoid with the aminoglycosides may be an alternative to potentiate the expected results in treatment against S. aureus and E. coli, since their association leads to a synergistic effect, reducing the MIC of these drugs and decreasing the dose necessary for therapeutic success.}, added-at = {2022-05-23T20:00:14.000+0200}, address = {24-28 OVAL RD, LONDON NW1 7DX, ENGLAND}, author = {Cruz, Beatriz G and dos Santos, Helcio S and Bandeira, Paulo N and Rodrigues, Tigressa Helena S and Matos, Maria Geysillene C and Nascimento, Mariana F and de Carvalho, Guilherme G C and Braz-Filho, Raimundo and Teixeira, Alexandre M R and Tintino, Saulo R and Coutinho, Henrique D M}, biburl = {https://www.bibsonomy.org/bibtex/24195e1de8401b78afefdb3f3f93167f2/ppgfis_ufc_br}, doi = {10.1016/j.micpath.2020.104144}, interhash = {7b6f6b792e950f901333c1b4d24b55bb}, intrahash = {4195e1de8401b78afefdb3f3f93167f2}, issn = {0882-4010}, journal = {MICROBIAL PATHOGENESIS}, keywords = {Antibacterial Multidrug-resistant activity; bacteria} kaempferol; {Flavonoid}, publisher = {ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD}, pubstate = {published}, timestamp = {2022-05-23T20:00:14.000+0200}, title = {Evaluation of antibacterial and enhancement of antibiotic action by the flavonoid kaempferol 7-O-beta-D-(6 `'-O-cumaroyl)-glucopyranoside isolated from Croton piauhiensis mull}, tppubtype = {article}, volume = 143, year = 2020 }