Under aerobic, high glucose conditions, Saccharomyces cerevisiae exhibits glucose repression and thus a predominantly fermentative metabolism. Here, we show that two commonly used prototrophic representatives of the CEN.PK and S288C strain families respond differently to deletion of the hexokinase 2 (HXK2) – a key player in glucose repression: In CEN.PK, growth rate collapses and derepression occurs on the physiological level, while the S288C descendant FY4 Δhxk2 still grows like the parent strain and shows a fully repressed metabolism. A CEN.PK Δhxk2 strain with a repaired adenylate cyclase gene CYR1 maintains repression but not growth rate. A comparison of the parent strain's physiology, metabolome, and proteome revealed higher metabolic rates, identical biomass, and byproduct yields, suggesting a lower Snf1 activity and a higher protein kinase A (PKA) activity in CEN.PK. This study highlights the importance of the genetic background in the processes of glucose signaling and regulation, contributes novel evidence on the overlap between the classical glucose repression pathway and the cAMP/PKA signaling pathway, and might have the potential to resolve some of the conflicting findings existing in the field.
%0 Journal Article
%1 Kummel2010Differential
%A Kümmel, Anne
%A Ewald, Jennifer C.
%A Fendt, Sarah-Maria
%A Jol, Stefan J.
%A Picotti, Paola
%A Aebersold, Ruedi
%A Sauer, Uwe
%A Zamboni, Nicola
%A Heinemann, Matthias
%D 2010
%I Blackwell Publishing Ltd
%J FEMS Yeast Research
%K glucose yeast
%N 3
%P 322--332
%R 10.1111/j.1567-1364.2010.00609.x
%T Differential glucose repression in common yeast strains in response to HXK2 deletion
%U http://dx.doi.org/10.1111/j.1567-1364.2010.00609.x
%V 10
%X Under aerobic, high glucose conditions, Saccharomyces cerevisiae exhibits glucose repression and thus a predominantly fermentative metabolism. Here, we show that two commonly used prototrophic representatives of the CEN.PK and S288C strain families respond differently to deletion of the hexokinase 2 (HXK2) – a key player in glucose repression: In CEN.PK, growth rate collapses and derepression occurs on the physiological level, while the S288C descendant FY4 Δhxk2 still grows like the parent strain and shows a fully repressed metabolism. A CEN.PK Δhxk2 strain with a repaired adenylate cyclase gene CYR1 maintains repression but not growth rate. A comparison of the parent strain's physiology, metabolome, and proteome revealed higher metabolic rates, identical biomass, and byproduct yields, suggesting a lower Snf1 activity and a higher protein kinase A (PKA) activity in CEN.PK. This study highlights the importance of the genetic background in the processes of glucose signaling and regulation, contributes novel evidence on the overlap between the classical glucose repression pathway and the cAMP/PKA signaling pathway, and might have the potential to resolve some of the conflicting findings existing in the field.
@article{Kummel2010Differential,
abstract = {Under aerobic, high glucose conditions, Saccharomyces cerevisiae exhibits glucose repression and thus a predominantly fermentative metabolism. Here, we show that two commonly used prototrophic representatives of the {CEN}.{PK} and {S288C} strain families respond differently to deletion of the hexokinase 2 ({HXK2}) – a key player in glucose repression: In {CEN}.{PK}, growth rate collapses and derepression occurs on the physiological level, while the {S288C} descendant {FY4} Δhxk2 still grows like the parent strain and shows a fully repressed metabolism. A {CEN}.{PK} Δhxk2 strain with a repaired adenylate cyclase gene {CYR1} maintains repression but not growth rate. A comparison of the parent strain's physiology, metabolome, and proteome revealed higher metabolic rates, identical biomass, and byproduct yields, suggesting a lower Snf1 activity and a higher protein kinase A ({PKA}) activity in {CEN}.{PK}. This study highlights the importance of the genetic background in the processes of glucose signaling and regulation, contributes novel evidence on the overlap between the classical glucose repression pathway and the {cAMP}/{PKA} signaling pathway, and might have the potential to resolve some of the conflicting findings existing in the field.},
added-at = {2018-12-02T16:09:07.000+0100},
author = {K\"{u}mmel, Anne and Ewald, Jennifer C. and Fendt, Sarah-Maria and Jol, Stefan J. and Picotti, Paola and Aebersold, Ruedi and Sauer, Uwe and Zamboni, Nicola and Heinemann, Matthias},
biburl = {https://www.bibsonomy.org/bibtex/2419ffd5c52393ed37e33b1ca4c80fe17/karthikraman},
citeulike-article-id = {6767360},
citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1567-1364.2010.00609.x},
citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/20199578},
citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=20199578},
day = 22,
doi = {10.1111/j.1567-1364.2010.00609.x},
interhash = {a1bb800178d9b254811b14adbe8f84d5},
intrahash = {419ffd5c52393ed37e33b1ca4c80fe17},
issn = {1567-1364},
journal = {FEMS Yeast Research},
keywords = {glucose yeast},
month = jan,
number = 3,
pages = {322--332},
pmid = {20199578},
posted-at = {2010-03-05 14:24:44},
priority = {5},
publisher = {Blackwell Publishing Ltd},
timestamp = {2018-12-02T16:09:07.000+0100},
title = {Differential glucose repression in common yeast strains in response to {HXK2} deletion},
url = {http://dx.doi.org/10.1111/j.1567-1364.2010.00609.x},
volume = 10,
year = 2010
}