%0 Journal Article %1 citeulike:470526 %A Liu, C. H. %A Liao, K. T. %A Huang, H. J. %C Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan. %D 2000 %J Anal Chem %K amperometry sandwich immunoassay immunosensor ag_detection electrochemistry immunoelectrode %N 13 %P 2925--2929 %R 10.1021/ac9914317 %T Amperometric immunosensors based on protein A coupled polyaniline-perfluorosulfonated ionomer composite electrodes. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10905329 %V 72 %X A very sensitive immunosensor based on polyaniline/ Nafion/protein A (PA/NF/PrA) composite electrodes has been developed for the amperometric immunoanalysis with urease-labeled immunoreagents. The use of urease conjugated goat anti-RIgG (GaRIgG-Ur) as the labeled antibody and urea as the substrate with an amperometric detection at -200 mV (vs Ag/AgCl) resulted in a dynamic range of 50-2000 ng mL-1 and a low detection limit of 10 ng/mL (64 pM) for the immunoanalysis of rabbit immunoglobulin G (RIgG). Because of the special affinity between protein A and RIgG, the PA/NF/PrA electrode can be regenerated repetitively by changing the pH of the buffer solutions. Characteristics of the PA/NF/PrA/RIgG immunosensor and optimal conditions for the competitive immunoanalysis of RIgG with FIA were studied.

@article{citeulike:470526, abstract = {A very sensitive immunosensor based on polyaniline/ Nafion/protein A (PA/NF/PrA) composite electrodes has been developed for the amperometric immunoanalysis with urease-labeled immunoreagents. The use of urease conjugated goat anti-RIgG (GaRIgG-Ur) as the labeled antibody and urea as the substrate with an amperometric detection at -200 mV (vs Ag/AgCl) resulted in a dynamic range of 50-2000 ng mL-1 and a low detection limit of 10 ng/mL (64 pM) for the immunoanalysis of rabbit immunoglobulin G (RIgG). Because of the special affinity between protein A and RIgG, the PA/NF/PrA electrode can be regenerated repetitively by changing the pH of the buffer solutions. Characteristics of the PA/NF/PrA/RIgG immunosensor and optimal conditions for the competitive immunoanalysis of RIgG with FIA were studied.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan.}, author = {Liu, C. H. and Liao, K. T. and Huang, H. J.}, biburl = {https://www.bibsonomy.org/bibtex/26c03a3547c820c8605fb10f76077e1f0/biblio24}, citeulike-article-id = {470526}, doi = {10.1021/ac9914317}, interhash = {6d2415599ae7a058ad5ec69817b5fd79}, intrahash = {6c03a3547c820c8605fb10f76077e1f0}, issn = {0003-2700}, journal = {Anal Chem}, keywords = {amperometry sandwich immunoassay immunosensor ag_detection electrochemistry immunoelectrode}, month = {July}, number = 13, pages = {2925--2929}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Amperometric immunosensors based on protein A coupled polyaniline-perfluorosulfonated ionomer composite electrodes.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=10905329}, volume = 72, year = 2000 }