%0 Journal Article %1 citeulike:470781 %A Keay, R. W. %A McNeil, C. J. %C Department of Clinical Biochemistry, Medical School, University of Newcastle Upon Tyne, UK. %D 1998 %J Biosens Bioelectron %K amperometry competition immunoassay homogeneous immunosensor ag_detection electrochemistry immunoelectrode %N 9 %P 963--970 %R 10.1016/S0956-5663(98)00008-6 %T Separation-free electrochemical immunosensor for rapid determination of atrazine. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9839385 %V 13 %X A separation-free electrochemical immunoassay method for the detection of the pesticide atrazine is described. The method developed is a competitive ELISA incorporating disposable screen printed horseradish peroxidase modified electrodes as the detector element in conjunction with single-use atrazine immuno-membranes. Screen printed carbon electrodes were prepared using carbon ink incorporating horseradish peroxidase. A monoclonal antibody for atrazine was immobilised onto Biodyne C membranes which were, in turn, placed over the electrode surface. The assay was based on competition for available binding sites between free atrazine and an atrazine-glucose oxidase conjugate prepared 'in-house'. In the presence of glucose, H2O2 formed by the conjugate was reduced by enzyme-channelling via the HRP electrode. The HRP was in turn re-reduced by a direct electron transfer mechanism at a potential of +50 mV Vs Ag/AgCl. Any H2O2 formed in the bulk solution by unbound atrazine-GOD conjugate was scavenged by excess catalase thus removing the requirement for a washing step. The performance of the method was compared with a commercial immunoassay kit for atrazine.

@article{citeulike:470781, abstract = {A separation-free electrochemical immunoassay method for the detection of the pesticide atrazine is described. The method developed is a competitive ELISA incorporating disposable screen printed horseradish peroxidase modified electrodes as the detector element in conjunction with single-use atrazine immuno-membranes. Screen printed carbon electrodes were prepared using carbon ink incorporating horseradish peroxidase. A monoclonal antibody for atrazine was immobilised onto Biodyne C membranes which were, in turn, placed over the electrode surface. The assay was based on competition for available binding sites between free atrazine and an atrazine-glucose oxidase conjugate prepared 'in-house'. In the presence of glucose, H2O2 formed by the conjugate was reduced by enzyme-channelling via the HRP electrode. The HRP was in turn re-reduced by a direct electron transfer mechanism at a potential of +50 mV Vs Ag/AgCl. Any H2O2 formed in the bulk solution by unbound atrazine-GOD conjugate was scavenged by excess catalase thus removing the requirement for a washing step. The performance of the method was compared with a commercial immunoassay kit for atrazine.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Department of Clinical Biochemistry, Medical School, University of Newcastle Upon Tyne, UK.}, author = {Keay, R. W. and McNeil, C. J.}, biburl = {https://www.bibsonomy.org/bibtex/28147d4596e9cb85c45d55e5262ecb29b/biblio24}, citeulike-article-id = {470781}, comment = {calum.mcneil@ncl.ac.uk}, doi = {10.1016/S0956-5663(98)00008-6}, interhash = {4f33a52f8c4c232a9e2b32cbd4234a74}, intrahash = {8147d4596e9cb85c45d55e5262ecb29b}, issn = {0956-5663}, journal = {Biosens Bioelectron}, keywords = {amperometry competition immunoassay homogeneous immunosensor ag_detection electrochemistry immunoelectrode}, month = {October}, number = 9, pages = {963--970}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Separation-free electrochemical immunosensor for rapid determination of atrazine.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=9839385}, volume = 13, year = 1998 }