Proinflammatory cytokines play a major role in the pathogenesis of meningococcal disease and their serum levels in patients are correlated with the outcome of infection. Dendritic cells initiate immunity against Neisseria meningitidis and are a major source of proinflammatory cytokines. Here we show that physical interaction of human DC with N. meningitidis via the class A scavenger receptor (SRA) modulates cytokine release by DC. Phagocytosis and uptake via SRA were shown to increase release of TNF-alpha, IL-1 beta and IL-6. In contrast, secretion of IL-8 is enhanced after recognition of N. meningitidis via SRA and not dependent on phagocytosis. Binding of N. meningitidis results in dephosphorylation of SRA but not in upregulation of SRA transcription. Unlike previously thought, not all meningococcal strains are recognized via SRA. A constitutively unencapsulated carriage isolate could be shown not to bind to SRA and induce proinflammatory cytokines independent of this receptor. In conclusion, recognition via SRA by dendritic cells is likely to play a central role in the immune response to N. meningitidis.
%0 Journal Article
%1 villwock_recognition_2008
%A Villwock, Andrea
%A Schmitt, Corinna
%A Schielke, Stephanie
%A Frosch, Matthias
%A Kurzai, Oliver
%D 2008
%J Microbes and Infection / Institut Pasteur
%K A, Bacterial Capsules, Cells, Class Cultured, Dendritic Factor-alpha Humans, Interleukins, Necrosis Neisseria Phagocytosis, Receptors, Scavenger Tumor ag_kurzai meningitidis,
%N 10-11
%P 1158--1165
%R 10.1016/j.micinf.2008.06.009
%T Recognition via the class A scavenger receptor modulates cytokine secretion by human dendritic cells after contact with Neisseria meningitidis
%U http://www.ncbi.nlm.nih.gov/pubmed/18640287
%V 10
%X Proinflammatory cytokines play a major role in the pathogenesis of meningococcal disease and their serum levels in patients are correlated with the outcome of infection. Dendritic cells initiate immunity against Neisseria meningitidis and are a major source of proinflammatory cytokines. Here we show that physical interaction of human DC with N. meningitidis via the class A scavenger receptor (SRA) modulates cytokine release by DC. Phagocytosis and uptake via SRA were shown to increase release of TNF-alpha, IL-1 beta and IL-6. In contrast, secretion of IL-8 is enhanced after recognition of N. meningitidis via SRA and not dependent on phagocytosis. Binding of N. meningitidis results in dephosphorylation of SRA but not in upregulation of SRA transcription. Unlike previously thought, not all meningococcal strains are recognized via SRA. A constitutively unencapsulated carriage isolate could be shown not to bind to SRA and induce proinflammatory cytokines independent of this receptor. In conclusion, recognition via SRA by dendritic cells is likely to play a central role in the immune response to N. meningitidis.
@article{villwock_recognition_2008,
abstract = {Proinflammatory cytokines play a major role in the pathogenesis of meningococcal disease and their serum levels in patients are correlated with the outcome of infection. Dendritic cells initiate immunity against Neisseria meningitidis and are a major source of proinflammatory cytokines. Here we show that physical interaction of human {DC} with N. meningitidis via the class A scavenger receptor {(SRA)} modulates cytokine release by {DC.} Phagocytosis and uptake via {SRA} were shown to increase release of {TNF-alpha,} {IL-1} beta and {IL-6.} In contrast, secretion of {IL-8} is enhanced after recognition of N. meningitidis via {SRA} and not dependent on phagocytosis. Binding of N. meningitidis results in dephosphorylation of {SRA} but not in upregulation of {SRA} transcription. Unlike previously thought, not all meningococcal strains are recognized via {SRA.} A constitutively unencapsulated carriage isolate could be shown not to bind to {SRA} and induce proinflammatory cytokines independent of this receptor. In conclusion, recognition via {SRA} by dendritic cells is likely to play a central role in the immune response to N. meningitidis.},
added-at = {2011-04-07T15:44:20.000+0200},
author = {Villwock, Andrea and Schmitt, Corinna and Schielke, Stephanie and Frosch, Matthias and Kurzai, Oliver},
biburl = {https://www.bibsonomy.org/bibtex/2b548e2855f23fbf3d301ddf6d259d31a/hymi},
doi = {10.1016/j.micinf.2008.06.009},
interhash = {02214ed37205b1c18ee9e9c5dcda02fc},
intrahash = {b548e2855f23fbf3d301ddf6d259d31a},
issn = {1286-4579},
journal = {Microbes and Infection / Institut Pasteur},
keywords = {A, Bacterial Capsules, Cells, Class Cultured, Dendritic Factor-alpha Humans, Interleukins, Necrosis Neisseria Phagocytosis, Receptors, Scavenger Tumor ag_kurzai meningitidis,},
month = sep,
note = {{PMID:} 18640287},
number = {10-11},
pages = {1158--1165},
timestamp = {2011-04-07T16:32:55.000+0200},
title = {Recognition via the class A scavenger receptor modulates cytokine secretion by human dendritic cells after contact with Neisseria meningitidis},
url = {http://www.ncbi.nlm.nih.gov/pubmed/18640287},
volume = 10,
year = 2008
}