Plants and animals employ innate immune systems to cope with microbial infection. Pattern-triggered immunity relies on the recognition of microbe-derived patterns by pattern recognition receptors (PRRs). Necrosis and ethylene-inducing peptide 1-like proteins (NLPs) constitute plant immunogenic patterns that are unique, as these proteins are produced by multiple prokaryotic (bacterial) and eukaryotic (fungal, oomycete) species. Here we show that the leucine-rich repeat receptor protein (LRR-RP) RLP23 binds in vivo to a conserved 20-amino-acid fragment found in most NLPs (nlp20), thereby mediating immune activation in Arabidopsis thaliana. RLP23 forms a constitutive, ligand-independent complex with the LRR receptor kinase (LRR-RK) SOBIR1 (Suppressor of Brassinosteroid insensitive 1 (BRI1)-associated kinase (BAK1)-interacting receptor kinase 1), and recruits a second LRR-RK, BAK1, into a tripartite complex upon ligand binding. Stable, ectopic expression of RLP23 in potato (Solanum tuberosum) confers nlp20 pattern recognition and enhanced immunity to destructive oomycete and fungal plant pathogens, such as Phytophthora infestans and Sclerotinia sclerotiorum. PRRs that recognize widespread microbial patterns might be particularly suited for engineering immunity in crop plants.
%0 Journal Article
%1 RN1070
%A Albert, I.
%A Böhm, H.
%A Albert, M.
%A Feiler, C. E.
%A Imkampe, J.
%A Wallmeroth, N.
%A Brancato, C.
%A Raaymakers, T. M.
%A Oome, S.
%A Zhang, H. Q.
%A Krol, E.
%A Grefen, C.
%A Gust, A. A.
%A Chai, J. J.
%A Hedrich, R.
%A Van den Ackerveken, G.
%A Nürnberger, T.
%D 2015
%J Nature Plants
%K myOwn pattern-recognition receptor
%N 10
%R Artn 15140
10.1038/Nplants.2015.140
%T An RLP23-SOBIR1-BAK1 complex mediates NLP-triggered immunity
%U /brokenurl#<Go to ISI>://WOS:000364414800001
%V 1
%X Plants and animals employ innate immune systems to cope with microbial infection. Pattern-triggered immunity relies on the recognition of microbe-derived patterns by pattern recognition receptors (PRRs). Necrosis and ethylene-inducing peptide 1-like proteins (NLPs) constitute plant immunogenic patterns that are unique, as these proteins are produced by multiple prokaryotic (bacterial) and eukaryotic (fungal, oomycete) species. Here we show that the leucine-rich repeat receptor protein (LRR-RP) RLP23 binds in vivo to a conserved 20-amino-acid fragment found in most NLPs (nlp20), thereby mediating immune activation in Arabidopsis thaliana. RLP23 forms a constitutive, ligand-independent complex with the LRR receptor kinase (LRR-RK) SOBIR1 (Suppressor of Brassinosteroid insensitive 1 (BRI1)-associated kinase (BAK1)-interacting receptor kinase 1), and recruits a second LRR-RK, BAK1, into a tripartite complex upon ligand binding. Stable, ectopic expression of RLP23 in potato (Solanum tuberosum) confers nlp20 pattern recognition and enhanced immunity to destructive oomycete and fungal plant pathogens, such as Phytophthora infestans and Sclerotinia sclerotiorum. PRRs that recognize widespread microbial patterns might be particularly suited for engineering immunity in crop plants.
@article{RN1070,
abstract = {Plants and animals employ innate immune systems to cope with microbial infection. Pattern-triggered immunity relies on the recognition of microbe-derived patterns by pattern recognition receptors (PRRs). Necrosis and ethylene-inducing peptide 1-like proteins (NLPs) constitute plant immunogenic patterns that are unique, as these proteins are produced by multiple prokaryotic (bacterial) and eukaryotic (fungal, oomycete) species. Here we show that the leucine-rich repeat receptor protein (LRR-RP) RLP23 binds in vivo to a conserved 20-amino-acid fragment found in most NLPs (nlp20), thereby mediating immune activation in Arabidopsis thaliana. RLP23 forms a constitutive, ligand-independent complex with the LRR receptor kinase (LRR-RK) SOBIR1 (Suppressor of Brassinosteroid insensitive 1 (BRI1)-associated kinase (BAK1)-interacting receptor kinase 1), and recruits a second LRR-RK, BAK1, into a tripartite complex upon ligand binding. Stable, ectopic expression of RLP23 in potato (Solanum tuberosum) confers nlp20 pattern recognition and enhanced immunity to destructive oomycete and fungal plant pathogens, such as Phytophthora infestans and Sclerotinia sclerotiorum. PRRs that recognize widespread microbial patterns might be particularly suited for engineering immunity in crop plants.},
added-at = {2024-02-14T14:38:32.000+0100},
author = {Albert, I. and Böhm, H. and Albert, M. and Feiler, C. E. and Imkampe, J. and Wallmeroth, N. and Brancato, C. and Raaymakers, T. M. and Oome, S. and Zhang, H. Q. and Krol, E. and Grefen, C. and Gust, A. A. and Chai, J. J. and Hedrich, R. and Van den Ackerveken, G. and Nürnberger, T.},
biburl = {https://www.bibsonomy.org/bibtex/2d996903fbf49bc9e94fe293cb62f2418/rainerhedrich_2},
doi = {Artn 15140
10.1038/Nplants.2015.140},
interhash = {1c2dd063de6d25b21fd4575818aa4eea},
intrahash = {d996903fbf49bc9e94fe293cb62f2418},
issn = {2055-026x},
journal = {Nature Plants},
keywords = {myOwn pattern-recognition receptor},
note = {Cv6xi
Times Cited:281
Cited References Count:53},
number = 10,
timestamp = {2024-02-14T14:38:32.000+0100},
title = {An RLP23-SOBIR1-BAK1 complex mediates NLP-triggered immunity},
type = {Journal Article},
url = {/brokenurl#<Go to ISI>://WOS:000364414800001},
volume = 1,
year = 2015
}