%0 Journal Article %1 citeulike:470485 %A Santandreu, M. %A Céspedes, F. %A Alegret, S. %A Martínez-Fàbregas, E. %C Departament de Química, Universitat Autònoma de Barcelona, Bellaterra, Catalonia, Spain. %D 1997 %J Anal Chem %K amperometry competition immunoassay immunosensor ag_detection electrochemistry immunoelectrode %N 11 %P 2080--2085 %R 10.1021/ac961222b %T Amperometric immunosensors based on rigid conducting immunocomposites. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9183175 %V 69 %X Novel polishable immunosensors based on rigid biocomposite materials have been constructed. These biocomposites contain graphite powder, rabbit IgG, and methacrylate or epoxy resins. This material acts as a reservoir for the biological molecules and as a transducer at the same time. In order to study the potential analytical properties of this new type of material, a competitive binding assay was developed to determine the RIgG present in a sample with the aid of goat anti-rabbit IgG labeled with alkaline phosphatase. Using phenyl phosphate as a substrate, the phenol produced by the enzymatic reaction was amperometrically detected at 800 mV (vs Ag/AgC1). The surface of the immunosensor can be regenerated by simply polishing, obtaining fresh immunocomposite ready to be used in a new competitive assay.

@article{citeulike:470485, abstract = {Novel polishable immunosensors based on rigid biocomposite materials have been constructed. These biocomposites contain graphite powder, rabbit IgG, and methacrylate or epoxy resins. This material acts as a reservoir for the biological molecules and as a transducer at the same time. In order to study the potential analytical properties of this new type of material, a competitive binding assay was developed to determine the RIgG present in a sample with the aid of goat anti-rabbit IgG labeled with alkaline phosphatase. Using phenyl phosphate as a substrate, the phenol produced by the enzymatic reaction was amperometrically detected at 800 mV (vs Ag/AgC1). The surface of the immunosensor can be regenerated by simply polishing, obtaining fresh immunocomposite ready to be used in a new competitive assay.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Departament de Química, Universitat Autònoma de Barcelona, Bellaterra, Catalonia, Spain.}, author = {Santandreu, M. and Céspedes, F. and Alegret, S. and Martínez-Fàbregas, E.}, biburl = {https://www.bibsonomy.org/bibtex/2e7c37a36b107771f9558ad749d0bf129/biblio24}, citeulike-article-id = {470485}, doi = {10.1021/ac961222b}, interhash = {9f0902ff206f782d242cd82cf2152d7a}, intrahash = {e7c37a36b107771f9558ad749d0bf129}, issn = {0003-2700}, journal = {Anal Chem}, keywords = {amperometry competition immunoassay immunosensor ag_detection electrochemistry immunoelectrode}, month = {June}, number = 11, pages = {2080--2085}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Amperometric immunosensors based on rigid conducting immunocomposites.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=9183175}, volume = 69, year = 1997 }