In previous studies, we have shown that annual expression profiles of cambial and wood tissue with respect to the Shaker K+ channel PTORK correlate with cambial activity. To follow PTORK-gene activity on the cellular level, we isolated the respective promoter regions and generated transgenic Arabidopsis plants expressing the GUS gene under the control of the PTORK promoter. Cross-sections of petioles showed PTORK-driven signals predominantly in the xylem parenchyma surrounding the vessels and in the phloem. Antibodies raised against a unique N-terminal region of PTORK in histo-immunochemical analyses recognised this K+ -release channel in growth-active poplar plants only. PTORK labelling was found in differentiating xylem cells (young fibres) and mature xylem (vessel-associated cells of the ray parenchyma). Patch-clamp measurements on fibre cell protoplasts, derived from young poplar twigs, identified outward-rectifying K+ channels as the major K+ conductance of this cell type, which resembled the biophysical properties of PTORK when expressed in Xenopus oocytes.
%0 Journal Article
%1 RN1174
%A Arend, M.
%A Stinzing, A.
%A Wind, C.
%A Langer, K.
%A Latz, A.
%A Ache, P.
%A Fromm, J.
%A Hedrich, R.
%D 2005
%J Planta
%K electrophysiology myOwn
%N 1
%P 140-148
%R 10.1007/s00425-005-0122-y
%T Polar-localised poplar K
channel capable of controlling electrical properties of wood-forming cells
%U /brokenurl#<Go to ISI>://WOS:000233725000014
%V 223
%X In previous studies, we have shown that annual expression profiles of cambial and wood tissue with respect to the Shaker K+ channel PTORK correlate with cambial activity. To follow PTORK-gene activity on the cellular level, we isolated the respective promoter regions and generated transgenic Arabidopsis plants expressing the GUS gene under the control of the PTORK promoter. Cross-sections of petioles showed PTORK-driven signals predominantly in the xylem parenchyma surrounding the vessels and in the phloem. Antibodies raised against a unique N-terminal region of PTORK in histo-immunochemical analyses recognised this K+ -release channel in growth-active poplar plants only. PTORK labelling was found in differentiating xylem cells (young fibres) and mature xylem (vessel-associated cells of the ray parenchyma). Patch-clamp measurements on fibre cell protoplasts, derived from young poplar twigs, identified outward-rectifying K+ channels as the major K+ conductance of this cell type, which resembled the biophysical properties of PTORK when expressed in Xenopus oocytes.
@article{RN1174,
abstract = {In previous studies, we have shown that annual expression profiles of cambial and wood tissue with respect to the Shaker K+ channel PTORK correlate with cambial activity. To follow PTORK-gene activity on the cellular level, we isolated the respective promoter regions and generated transgenic Arabidopsis plants expressing the GUS gene under the control of the PTORK promoter. Cross-sections of petioles showed PTORK-driven signals predominantly in the xylem parenchyma surrounding the vessels and in the phloem. Antibodies raised against a unique N-terminal region of PTORK in histo-immunochemical analyses recognised this K+ -release channel in growth-active poplar plants only. PTORK labelling was found in differentiating xylem cells (young fibres) and mature xylem (vessel-associated cells of the ray parenchyma). Patch-clamp measurements on fibre cell protoplasts, derived from young poplar twigs, identified outward-rectifying K+ channels as the major K+ conductance of this cell type, which resembled the biophysical properties of PTORK when expressed in Xenopus oocytes.},
added-at = {2024-02-14T14:38:32.000+0100},
author = {Arend, M. and Stinzing, A. and Wind, C. and Langer, K. and Latz, A. and Ache, P. and Fromm, J. and Hedrich, R.},
biburl = {https://www.bibsonomy.org/bibtex/2e92c669315e619547fd04e2703fa29a7/rainerhedrich_2},
doi = {10.1007/s00425-005-0122-y},
interhash = {ec5157e21369e481618c26fc5dc76a27},
intrahash = {e92c669315e619547fd04e2703fa29a7},
issn = {0032-0935},
journal = {Planta},
keywords = {electrophysiology myOwn},
note = {990ec
Times Cited:21
Cited References Count:38},
number = 1,
pages = {140-148},
timestamp = {2024-02-14T14:38:32.000+0100},
title = {Polar-localised poplar K
channel capable of controlling electrical properties of wood-forming cells},
type = {Journal Article},
url = {/brokenurl#<Go to ISI>://WOS:000233725000014},
volume = 223,
year = 2005
}