%0 Journal Article %1 Mlodzianoski2018 %A Mlodzianoski, Michael J. %A Cheng-Hathaway, Paul J. %A Bemiller, Shane M. %A McCray, Tyler J. %A Liu, Sheng %A Miller, David A. %A Lamb, Bruce T. %A Landreth, Gary E. %A Huang, Fang %D 2018 %J Nature Methods %K 3D adaptive_optics imaging microscopy psf_engineering smlm superresolution %N 8 %P 583--586 %R 10.1038/s41592-018-0053-8 %T Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections %V 15 %X Application of single-molecule switching nanoscopy (SMSN) beyond the coverslip surface poses substantial challenges due to sample-induced aberrations that distort and blur single-molecule emission patterns. We combined active shaping of point spread functions and efficient adaptive optics to enable robust 3D-SMSN imaging within tissues. This development allowed us to image through 30-$\mu$m-thick brain sections to visualize and reconstruct the morphology and the nanoscale details of amyloid-$\beta$ filaments in a mouse model of Alzheimer's disease.

@article{Mlodzianoski2018, abstract = {Application of single-molecule switching nanoscopy (SMSN) beyond the coverslip surface poses substantial challenges due to sample-induced aberrations that distort and blur single-molecule emission patterns. We combined active shaping of point spread functions and efficient adaptive optics to enable robust 3D-SMSN imaging within tissues. This development allowed us to image through 30-$\mu$m-thick brain sections to visualize and reconstruct the morphology and the nanoscale details of amyloid-$\beta$ filaments in a mouse model of Alzheimer's disease.}, added-at = {2020-04-06T13:11:22.000+0200}, author = {Mlodzianoski, Michael J. and Cheng-Hathaway, Paul J. and Bemiller, Shane M. and McCray, Tyler J. and Liu, Sheng and Miller, David A. and Lamb, Bruce T. and Landreth, Gary E. and Huang, Fang}, biburl = {https://www.bibsonomy.org/bibtex/2ee3808cb1c40d1e3a88b6df685c91b0d/kfriedl}, doi = {10.1038/s41592-018-0053-8}, file = {:C$\backslash$:/Users/Karoline/AppData/Local/Mendeley Ltd/Mendeley Desktop/Downloaded/mlodzianoski.pdf:pdf}, interhash = {01f779ff639d3b1e441b057150b2a020}, intrahash = {ee3808cb1c40d1e3a88b6df685c91b0d}, issn = {15487105}, journal = {Nature Methods}, keywords = {3D adaptive_optics imaging microscopy psf_engineering smlm superresolution}, number = 8, pages = {583--586}, timestamp = {2020-04-06T14:08:03.000+0200}, title = {{Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections}}, volume = 15, year = 2018 }