%0 Journal Article %1 Almada2019 %A Almada, Pedro %A Pereira, Pedro M. %A Culley, Siân %A Caillol, Ghislaine %A Boroni-Rueda, Fanny %A Dix, Christina L. %A Charras, Guillaume %A Baum, Buzz %A Laine, Romain F. %A Leterrier, Christophe %A Henriques, Ricardo %D 2019 %J Nature Communications %K automated exchange multicolor multiplexing paint pumpy sequential superresolution team %N 1 %R 10.1038/s41467-019-09231-9 %T Automating multimodal microscopy with NanoJ-Fluidics %U https://doi.org/10.1038/s41467-019-09231-9 %V 10 %X Combining and multiplexing microscopy approaches is crucial to understand cellular events, but requires elaborate workflows. Here, we present a robust, open-source approach for treating, labelling and imaging live or fixed cells in automated sequences. NanoJ-Fluidics is based on low-cost Lego hardware controlled by ImageJ-based software, making high-content, multimodal imaging easy to implement on any microscope with high reproducibility. We demonstrate its capacity on event-driven, super-resolved live-to-fixed and multiplexed STORM/DNA-PAINT experiments.

@article{Almada2019, abstract = {Combining and multiplexing microscopy approaches is crucial to understand cellular events, but requires elaborate workflows. Here, we present a robust, open-source approach for treating, labelling and imaging live or fixed cells in automated sequences. NanoJ-Fluidics is based on low-cost Lego hardware controlled by ImageJ-based software, making high-content, multimodal imaging easy to implement on any microscope with high reproducibility. We demonstrate its capacity on event-driven, super-resolved live-to-fixed and multiplexed STORM/DNA-PAINT experiments.}, added-at = {2020-03-23T21:12:34.000+0100}, author = {Almada, Pedro and Pereira, Pedro M. and Culley, Si{\^{a}}n and Caillol, Ghislaine and Boroni-Rueda, Fanny and Dix, Christina L. and Charras, Guillaume and Baum, Buzz and Laine, Romain F. and Leterrier, Christophe and Henriques, Ricardo}, biburl = {https://www.bibsonomy.org/bibtex/2f78ce9a402b71a066cc68f4fcdeec2c3/kfriedl}, doi = {10.1038/s41467-019-09231-9}, file = {:C$\backslash$:/Users/Karoline/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Almada et al. - Unknown - Automating multimodal microscopy with NanoJ-Fluidics.pdf:pdf}, interhash = {e3e88f5a8651433fc679a2cd9a210987}, intrahash = {f78ce9a402b71a066cc68f4fcdeec2c3}, issn = {20411723}, journal = {Nature Communications}, keywords = {automated exchange multicolor multiplexing paint pumpy sequential superresolution team}, number = 1, timestamp = {2020-04-07T11:02:24.000+0200}, title = {{Automating multimodal microscopy with NanoJ-Fluidics}}, url = {https://doi.org/10.1038/s41467-019-09231-9}, volume = 10, year = 2019 }