In vitro studies of magnesium oxide nano-particles of Saccharum officinarum root extract: Evaluation of the antidiabetic effect using alpha-glucosidase enzyme
World Journal of Biology Pharmacy and Health Sciences, 16 (1):
117–122 (March 2024)DOI: 10.30574/wjbphs.2023.16.1.0357
Abstract
Objective: Magnesium oxide nanoparticle green synthesis is said to have a wide range of biomedical uses. Inhibiting the activity of pancreatic enzymes is one method for treating diabetes. The goal of this study was to show in-vitro evidence that Saccharum officinarum magnesium oxide nanoparticles may inhibit the activity of the enzyme α-glucosidase. This study had a therapeutic focus as this could be emerging candidates of new drugs for controlling hyperglycemia.
Materials and Procedures: Saccharum officinarum root extract was used to create magnesium oxide nanoparticles, which were then examined for bioactive components and tested for α-glucosidase inhibition. When the enzyme substrate solution was incubated with various doses of the grass-root Magnesium oxide nanoparticles, the activity of the enzyme was monitored to identify the kind of inhibition.
Result: Magnesium oxide nanoparticles synthesized from Saccharum officinarum root extract showed concentration and time-dependent inhibition of α-glucosidase. An 85% inhibition was observed with 0.6 mg/ml of the synthesized nanoparticles.
Conclusion: The results showed that the ethanolic extract of MgO nanoparticles synthesized by Saccharum officinarum exhibited effective antidiabetic activity, supporting its use in the treatment and control of diabetes.