%0 Report %1 Huang2006 %A Huang, Zhen-Li %A Zhu, Hongyu %A Long, Fan %A Ma, Hongqiang %A Qin, Lingsong %A Liu, Yongfeng %A Ding, Jiuping %A Zhang, Zhihong %A Luo, Qingming %A Zeng, Shaoqun %A Michalet, X %A W Siegmund, O H %A Vallerga, J V %A Jelinsky, P %A Millaud, J E %A Weiss, S %A Betzig, E %A Patterson, G H %A Sougrat, R %A Lindwasser, O W %A Olenych, S %A Bonifacino, J S %A Davidson, M W %A Lippincott-Schwartz, J %A Hess, H F %A Fowler, B %A Liu, C A %A Mims, S %A Balicki, J %A Li, W %A Do, H %A Appelbaum, J %A Vu, P %A Quan, T W %A Zeng, S Q %A Huang, Z L %B Nat. Rev. Mol. Cell Biol %D 2006 %K microscopy smlm storm superresolution %N 5793 %P 258--272 %T Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM) %V 313 %X In the community of localization-based super-resolution microscopy (or called localization microscopy), it is generally believed that the emission of single molecules is so weak that an EMCCD (electron multiplying charge coupled device) camera is necessary to be used as the detector by eliminating read noise. Here we evaluate the possibility of a new kind of low light detector, scientific complementary metal-oxide-semiconductor (sCMOS) camera in localization microscopy. We demonstrate experimentally that sCMOS is capable of imaging actin bundles with FWHM diameter of 37 nm, evidencing the capability of sCMOS in localization microscopy. We further characterize the noise performance of sCMOS and find out that, with the use of a bright fluorescence probe such as d2EosFP, localization microscopy imaging is now working in the shot noise limited region., Ä 5.5Mpixel 100 frames/sec wide dynamic range low noise CMOS image sensor for scientific applications," Proc. SPIE 7536, 753607, 753607-12 (2010)., "Localization capability and limitation of electron-multiplying charge-coupled, scientific complementary metal-oxide semiconductor, and charge-coupled devices for superresolution imaging," J. Biomed. Opt. 15(6), 066005 (2010). 12. K. I. Mortensen, L. S. Churchman, J. A. Spudich, and H. Flyvbjerg, Öptimized localization analysis for single-molecule tracking and super-resolution microscopy,

@techreport{Huang2006, abstract = {In the community of localization-based super-resolution microscopy (or called localization microscopy), it is generally believed that the emission of single molecules is so weak that an EMCCD (electron multiplying charge coupled device) camera is necessary to be used as the detector by eliminating read noise. Here we evaluate the possibility of a new kind of low light detector, scientific complementary metal-oxide-semiconductor (sCMOS) camera in localization microscopy. We demonstrate experimentally that sCMOS is capable of imaging actin bundles with FWHM diameter of 37 nm, evidencing the capability of sCMOS in localization microscopy. We further characterize the noise performance of sCMOS and find out that, with the use of a bright fluorescence probe such as d2EosFP, localization microscopy imaging is now working in the shot noise limited region., "A 5.5Mpixel 100 frames/sec wide dynamic range low noise CMOS image sensor for scientific applications," Proc. SPIE 7536, 753607, 753607-12 (2010)., "Localization capability and limitation of electron-multiplying charge-coupled, scientific complementary metal-oxide semiconductor, and charge-coupled devices for superresolution imaging," J. Biomed. Opt. 15(6), 066005 (2010). 12. K. I. Mortensen, L. S. Churchman, J. A. Spudich, and H. Flyvbjerg, "Optimized localization analysis for single-molecule tracking and super-resolution microscopy,}, added-at = {2020-04-06T13:11:22.000+0200}, author = {Huang, Zhen-Li and Zhu, Hongyu and Long, Fan and Ma, Hongqiang and Qin, Lingsong and Liu, Yongfeng and Ding, Jiuping and Zhang, Zhihong and Luo, Qingming and Zeng, Shaoqun and Michalet, X and {W Siegmund}, O H and Vallerga, J V and Jelinsky, P and Millaud, J E and Weiss, S and Betzig, E and Patterson, G H and Sougrat, R and Lindwasser, O W and Olenych, S and Bonifacino, J S and Davidson, M W and Lippincott-Schwartz, J and Hess, H F and Fowler, B and Liu, C A and Mims, S and Balicki, J and Li, W and Do, H and Appelbaum, J and Vu, P and Quan, T W and Zeng, S Q and Huang, Z L}, biburl = {https://www.bibsonomy.org/bibtex/24c5e2bf2895f566fb935ae679a307f51/kfriedl}, booktitle = {Nat. Rev. Mol. Cell Biol}, file = {::}, interhash = {31c224cedb994eaab0d98dc370d94d04}, intrahash = {4c5e2bf2895f566fb935ae679a307f51}, keywords = {microscopy smlm storm superresolution}, number = 5793, pages = {258--272}, timestamp = {2020-04-06T13:46:23.000+0200}, title = {{Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)}}, volume = 313, year = 2006 }