Zusammenfassung
We report a new and efficient methodology of labelling red blood cells,
in order to investigate the expression of anti-A antigen, employing
luminescent semiconductor nanocrystals. Highly luminescent and stable
core-shell cadmium sulphide/cadmium hydroxide CdS/CdS(OH)(2)
colloidal particles were obtained in the nanometre size range. The
surface of these particles was characterized by using a monoclonal
anti-A antibody via a one-step glutaraldehyde cross-linking procedure,
followed by conjugation of the particles to red cells of blood groups
A(+), A(2)(+) and O+. Laser scanning confocal microscopy images
indicated that after conjugation for 30 min, A(+) and A(2)(+)
erythrocytes presented different patterns of dual bright emission
whereas the O+ group cells showed no emission. We suggest that this
labelling procedure may be applied as a quantitative tool to investigate
the distribution and expression of alloantigen in red blood cells.
Nutzer