%0 Journal Article %1 triblMultidimensionalLiquidChromatography2008 %A Tribl, Florian %A Lohaus, Christiane %A Dombert, Tanja %A Langenfeld, Elmar %A Piechura, Heike %A Warscheid, Bettina %A Meyer, Helmut E. %A Marcus, Katrin %C Germany %D 2008 %J Proteomics %K & Chromatography Dyes/*chemistry,Isotope Gel,Fluorescence,Fluorescent High Labeling/*methods,Isotopes/chemistry,Mass Liquid/*methods,Electrophoresis Polyacrylamide Pressure Results,to_read Spectrometry,Proteome/analysis/chemistry/*isolation of purification,Proteomics/*methods,Reproducibility %N 6 %P 1204--1211 %R 10.1002/pmic.200700736 %T Towards Multidimensional Liquid Chromatography Separation of Proteins Using Fluorescence and Isotope-Coded Protein Labelling for Quantitative Proteomics. %V 8 %X HPLC has emerged as a valuable tool for separating proteins. To address the analysis of complex proteomes and quantitative changes of proteins therein, we developed a multidimensional LC (MDLC)-based approach followed by large gel 1-D SDS-PAGE. Here we present a novel strategy that allows for simultaneously identifying and quantifying differentially regulated proteins following three separation and fractionation steps. This MDLC platform integrates advantages of dual protein labelling using both fluorescence and isotope-coded tags for subsequent detection and quantification of abundance ratios of proteins by MS.

@article{triblMultidimensionalLiquidChromatography2008, abstract = {HPLC has emerged as a valuable tool for separating proteins. To address the analysis of complex proteomes and quantitative changes of proteins therein, we developed a multidimensional LC (MDLC)-based approach followed by large gel 1-D SDS-PAGE. Here we present a novel strategy that allows for simultaneously identifying and quantifying differentially regulated proteins following three separation and fractionation steps. This MDLC platform integrates advantages of dual protein labelling using both fluorescence and isotope-coded tags for subsequent detection and quantification of abundance ratios of proteins by MS.}, added-at = {2024-05-17T13:01:35.000+0200}, address = {Germany}, author = {Tribl, Florian and Lohaus, Christiane and Dombert, Tanja and Langenfeld, Elmar and Piechura, Heike and Warscheid, Bettina and Meyer, Helmut E. and Marcus, Katrin}, biburl = {https://www.bibsonomy.org/bibtex/2f8b141b386d16aa507715329efb8d6c8/warscheidlab}, doi = {10.1002/pmic.200700736}, interhash = {a02d7102ec2b86e3a1d7b3c8457b9807}, intrahash = {f8b141b386d16aa507715329efb8d6c8}, issn = {1615-9861 1615-9853}, journal = {Proteomics}, keywords = {& Chromatography Dyes/*chemistry,Isotope Gel,Fluorescence,Fluorescent High Labeling/*methods,Isotopes/chemistry,Mass Liquid/*methods,Electrophoresis Polyacrylamide Pressure Results,to_read Spectrometry,Proteome/analysis/chemistry/*isolation of purification,Proteomics/*methods,Reproducibility}, langid = {english}, month = mar, number = 6, pages = {1204--1211}, pmid = {18271069}, timestamp = {2024-05-17T13:01:35.000+0200}, title = {Towards Multidimensional Liquid Chromatography Separation of Proteins Using Fluorescence and Isotope-Coded Protein Labelling for Quantitative Proteomics.}, volume = 8, year = 2008 }