%0 Journal Article %1 citeulike:471080 %A Kröger, S. %A Setford, S. J. %A Turner, A. P. %C Cranfield Biotechnology Centre, Cranfield University, Bedfordshire, U.K. %D 1998 %J Anal Chem %K amperometry competition immunoassay ag_detection electrochemistry %N 23 %P 5047--5053 %R 10.1021/ac9805100 %T Immunosensor for 2,4-dichlorophenoxyacetic acid in aqueous/organic solvent soil extracts. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9852786 %V 70 %X The development of a simple electrochemical immunoassay procedure for the field-based quantification of the herbicide 2,4-D in methanolic soil extracts is presented. The sensor utilizes a competitive immunoassay format incorporating an immobilized antigen complex at the surface of a disposable screen-printed working electrode element. The extent of glucose oxidase-labeled antibody binding to the antigen--electrode is determined amperometrically and is related to sample analyte concentration. The performance of the sensor is assessed in buffer, 30% methanol, and methanolic soil extracts. The device is capable of quantifying 2,4-D in all three matrixes at the low ppm level with coefficient of variation values of 6.2-33.6%. The causes of the variation observed in the sensor response in different soil matrixes are examined and improvements proposed. The sensor, tested in parallel with a commercial 2,4-D immunoassay test kit, yields comparable quantitative data and detection limits while exhibiting greater assay simplicity.

@article{citeulike:471080, abstract = {The development of a simple electrochemical immunoassay procedure for the field-based quantification of the herbicide 2,4-D in methanolic soil extracts is presented. The sensor utilizes a competitive immunoassay format incorporating an immobilized antigen complex at the surface of a disposable screen-printed working electrode element. The extent of glucose oxidase-labeled antibody binding to the antigen--electrode is determined amperometrically and is related to sample analyte concentration. The performance of the sensor is assessed in buffer, 30% methanol, and methanolic soil extracts. The device is capable of quantifying 2,4-D in all three matrixes at the low ppm level with coefficient of variation values of 6.2-33.6%. The causes of the variation observed in the sensor response in different soil matrixes are examined and improvements proposed. The sensor, tested in parallel with a commercial 2,4-D immunoassay test kit, yields comparable quantitative data and detection limits while exhibiting greater assay simplicity.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Cranfield Biotechnology Centre, Cranfield University, Bedfordshire, U.K.}, author = {Kröger, S. and Setford, S. J. and Turner, A. P.}, biburl = {https://www.bibsonomy.org/bibtex/280305102d6b7f68e841c64bf5165fdce/biblio24}, citeulike-article-id = {471080}, doi = {10.1021/ac9805100}, interhash = {a38b6bfdcffdd410580dba23cbfe8366}, intrahash = {80305102d6b7f68e841c64bf5165fdce}, issn = {0003-2700}, journal = {Anal Chem}, keywords = {amperometry competition immunoassay ag_detection electrochemistry}, month = {December}, number = 23, pages = {5047--5053}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Immunosensor for 2,4-dichlorophenoxyacetic acid in aqueous/organic solvent soil extracts.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=9852786}, volume = 70, year = 1998 }