%0 Journal Article %1 citeulike:470524 %A Kelly, S. %A Compagnone, D. %A Guilbault, G. %C Chemistry Department, University College Cork, Ireland. %D 1998 %J Biosens Bioelectron %K amperometry lactate_dehydrogenase immunoassay immunosensor ag_detection electrochemistry immunoelectrode %N 2 %P 173--179 %R 10.1016/S0956-5663(97)00105-X %T Amperometric immunosensor for lactate dehydrogenase LD-1. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9597734 %V 13 %X An amperometric immunosensor for the detection of the lactate dehydrogenase isoenzyme LD-1 has been developed. Polyclonal antibodies for LD-1 have been covalently immobilised onto a preactivated Immunodyne ABC membrane, reacted with standard LD-1 solutions and placed onto a platinum working electrode polarised at +600 mV vs. Ag/AgCl. Lactate dehydrogenase activity has been measured by detection of the oxidation of NADH at the electrode surface. A calibration curve for LDH in the 0.005-0.12 U range has been obtained with a repeatability of 3% and a reproducibility of 10%. The probe exhibited good selectivity (response of LD-2 was 18% of LD-1) which was further improved using thermal treatment of the membranes. Measurement of LD-1 content in human control serum with the developed procedure gave a LD-1 concentration in the reported assigned range.

@article{citeulike:470524, abstract = {An amperometric immunosensor for the detection of the lactate dehydrogenase isoenzyme LD-1 has been developed. Polyclonal antibodies for LD-1 have been covalently immobilised onto a preactivated Immunodyne ABC membrane, reacted with standard LD-1 solutions and placed onto a platinum working electrode polarised at +600 mV vs. Ag/AgCl. Lactate dehydrogenase activity has been measured by detection of the oxidation of NADH at the electrode surface. A calibration curve for LDH in the 0.005-0.12 U range has been obtained with a repeatability of 3% and a reproducibility of 10%. The probe exhibited good selectivity (response of LD-2 was 18% of LD-1) which was further improved using thermal treatment of the membranes. Measurement of LD-1 content in human control serum with the developed procedure gave a LD-1 concentration in the reported assigned range.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Chemistry Department, University College Cork, Ireland.}, author = {Kelly, S. and Compagnone, D. and Guilbault, G.}, biburl = {https://www.bibsonomy.org/bibtex/2db2f6d5ec80243ade803b33acd2c4368/biblio24}, citeulike-article-id = {470524}, doi = {10.1016/S0956-5663(97)00105-X}, interhash = {dc421602c45b85eeaa85cfa0546fd542}, intrahash = {db2f6d5ec80243ade803b33acd2c4368}, issn = {0956-5663}, journal = {Biosens Bioelectron}, keywords = {amperometry lactate_dehydrogenase immunoassay immunosensor ag_detection electrochemistry immunoelectrode}, month = {February}, number = 2, pages = {173--179}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Amperometric immunosensor for lactate dehydrogenase LD-1.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=9597734}, volume = 13, year = 1998 }