Article,

Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton

, , and .
Nature Methods, 9 (2): 185--188 (February 2012)
DOI: 10.1038/nmeth.1841

Abstract

By combining astigmatism imaging with a dual-objective scheme, we improved the image resolution of stochastic optical reconstruction microscopy (STORM) and obtained \textless10-nm lateral resolution and \textless20-nm axial resolution when imaging biological specimens. Using this approach, we resolved individual actin filaments in cells and revealed three-dimensional ultrastructure of the actin cytoskeleton. We observed two vertically separated layers of actin networks with distinct structural organizations in sheet-like cell protrusions. © 2012 Nature America, Inc. All rights reserved.

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