%0 Journal Article %1 Piccirilli2010 %A Piccirilli, Gisela N. %A Escandar, Graciela M. %D 2010 %I The Royal Society of Chemistry %J Analyst %K fluorescence sensing spectrometer %P 1299-1308 %R 10.1039/b923565e %T Second-order advantage with excitation-emission fluorescence spectroscopy and a flow-through optosensing device. Simultaneous determination of thiabendazole and fuberidazole in the presence of uncalibrated interferences %U http://dx.doi.org/10.1039/b923565e %V 135 %X This paper presents a novel approach for the simultaneous determination of two widely used fungicides in a very interfering environment, combining the advantage of a spectrofluorimetric optosensor coupled to a flow-injection system and the selectivity of second-order chemometric algorithms. The sensor is based on the simultaneous retention of thiabendazole and fuberidazole on C18-bonded phase placed inside a flow-cell. After the arrival of the analytes to the sensing zone, the flow is stopped and the excitation-emission fluorescence matrix is read in a fast-scanning spectrofluorimeter. Parallel factor analysis (PARAFAC) and unfolded and multidimensional partial least-squares coupled to residual bilinearization (U- and N-PLS/RBL) were selected for data processing. These algorithms achieve the second-order advantage, and are in principle able to overcome the problem of the presence of unexpected interferences. The power of U-PLS/RBL to quantify both fungicides at parts-per-billion levels, even in the presence of high concentrations of spectral interferences such as carbaryl, carbendazim and 1-naphthylacetic acid, is demonstrated. Indeed, U-PLS/RBL allowed us to reach selectivity using a commercial but non-selective sensing support. To the best of our knowledge, this is the first time the potentiality of the 'second-order advantage' is evaluated on a flow-injection system, using an unspecific supporting material and in the presence of three real interferences. Using a sample volume of 2 mL, detection limits of 4 ng mL-1 and 0.3 ng mL-1 for thiabendazole and fuberidazol were respectively obtained in samples without interferences. In samples containing interferences, the limits of detection were 17 and 1 ng mL-1 for thiabendazole and fuberidazol, respectively. The sample frequency, including excitation/emission fluorescence matrix measurements, was 12 samples h-1. The sensor was satisfactorily applied to the determination of both analytes in real water samples.

@article{Piccirilli2010, abstract = {This paper presents a novel approach for the simultaneous determination of two widely used fungicides in a very interfering environment, combining the advantage of a spectrofluorimetric optosensor coupled to a flow-injection system and the selectivity of second-order chemometric algorithms. The sensor is based on the simultaneous retention of thiabendazole and fuberidazole on C18-bonded phase placed inside a flow-cell. After the arrival of the analytes to the sensing zone, the flow is stopped and the excitation-emission fluorescence matrix is read in a fast-scanning spectrofluorimeter. Parallel factor analysis (PARAFAC) and unfolded and multidimensional partial least-squares coupled to residual bilinearization (U- and N-PLS/RBL) were selected for data processing. These algorithms achieve the second-order advantage, and are in principle able to overcome the problem of the presence of unexpected interferences. The power of U-PLS/RBL to quantify both fungicides at parts-per-billion levels, even in the presence of high concentrations of spectral interferences such as carbaryl, carbendazim and 1-naphthylacetic acid, is demonstrated. Indeed, U-PLS/RBL allowed us to reach selectivity using a commercial but non-selective sensing support. To the best of our knowledge, this is the first time the potentiality of the 'second-order advantage' is evaluated on a flow-injection system, using an unspecific supporting material and in the presence of three real interferences. Using a sample volume of 2 mL, detection limits of 4 ng mL-1 and 0.3 ng mL-1 for thiabendazole and fuberidazol were respectively obtained in samples without interferences. In samples containing interferences, the limits of detection were 17 and 1 ng mL-1 for thiabendazole and fuberidazol, respectively. The sample frequency, including excitation/emission fluorescence matrix measurements, was 12 samples h-1. The sensor was satisfactorily applied to the determination of both analytes in real water samples.}, added-at = {2011-10-01T01:01:09.000+0200}, author = {Piccirilli, Gisela N. and Escandar, Graciela M.}, biburl = {https://www.bibsonomy.org/bibtex/279c3ba750ac264f75c9bbba73449d70e/afcallender}, citeulike-article-id = {7034023}, citeulike-linkout-0 = {http://dx.doi.org/10.1039/b923565e}, citeulike-linkout-1 = {http://www.rsc.org/Publishing/Journals/article.asp?doi=b923565e}, doi = {10.1039/b923565e}, file = {Piccirilli2010.pdf:indexed\\Piccirilli2010.pdf:PDF}, groups = {public}, interhash = {e14413f5ba99827d5c5d626aa7a907ee}, intrahash = {79c3ba750ac264f75c9bbba73449d70e}, journal = {Analyst}, keywords = {fluorescence sensing spectrometer}, pages = {1299-1308}, posted-at = {2010-04-19 02:14:47}, priority = {5}, publisher = {The Royal Society of Chemistry}, timestamp = {2011-10-01T01:01:09.000+0200}, title = {Second-order advantage with excitation-emission fluorescence spectroscopy and a flow-through optosensing device. Simultaneous determination of thiabendazole and fuberidazole in the presence of uncalibrated interferences}, url = {http://dx.doi.org/10.1039/b923565e}, username = {afcallender}, volume = 135, year = 2010 }