%0 Journal Article %1 citeulike:467034 %A Kubotsu, K. %A Goto, S. %A Fujita, M. %A Tuchiya, H. %A Kida, M. %A Takano, S. %A Matsuura, S. %A Sakurabayashi, I. %C Osaka Research Laboratory, Wako Pure Chemical Industries, Ltd., Japan. %D 1992 %J Clin Chem %K l-i-l-a automated complement immunoassay homogeneous ag_detection %N 6 %P 808--812 %T Automated homogeneous liposome immunoassay systems for anticonvulsant drugs. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1597006 %V 38 %X We developed automated homogeneous immunoassays, based on immunolysis of liposomes, for measuring phenytoin, phenobarbital, and carbamazepine from serum. Liposome lysis was detected spectrophotometrically from entrapped glucose-6-phosphate dehydrogenase activity. The procedure was fully automated on a routine automated clinical analyzer. Within-run, between-run, dilution, and recovery tests showed good accuracies and reproducibilities. Bilirubin, hemoglobin, triglycerides, and Intrafat did not affect assay results. The results obtained by liposome immunoassays for phenytoin, phenobarbital, and carbamazepine correlated well with those obtained by enzyme-multiplied immunoassay (Syva EMIT) kits (r = 0.995, 0.986, and 0.988, respectively) and fluorescence polarization immunoassay (Abbott TDx) kits (r = 0.990, 0.991, and 0.975, respectively). The proposed method should be useful for monitoring anticonvulsant drug concentrations in blood.

@article{citeulike:467034, abstract = {We developed automated homogeneous immunoassays, based on immunolysis of liposomes, for measuring phenytoin, phenobarbital, and carbamazepine from serum. Liposome lysis was detected spectrophotometrically from entrapped glucose-6-phosphate dehydrogenase activity. The procedure was fully automated on a routine automated clinical analyzer. Within-run, between-run, dilution, and recovery tests showed good accuracies and reproducibilities. Bilirubin, hemoglobin, triglycerides, and Intrafat did not affect assay results. The results obtained by liposome immunoassays for phenytoin, phenobarbital, and carbamazepine correlated well with those obtained by enzyme-multiplied immunoassay (Syva EMIT) kits (r = 0.995, 0.986, and 0.988, respectively) and fluorescence polarization immunoassay (Abbott TDx) kits (r = 0.990, 0.991, and 0.975, respectively). The proposed method should be useful for monitoring anticonvulsant drug concentrations in blood.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Osaka Research Laboratory, Wako Pure Chemical Industries, Ltd., Japan.}, author = {Kubotsu, K. and Goto, S. and Fujita, M. and Tuchiya, H. and Kida, M. and Takano, S. and Matsuura, S. and Sakurabayashi, I.}, biburl = {https://www.bibsonomy.org/bibtex/2ca1d616e2aedce2b7ac619764dd6fc50/biblio24}, citeulike-article-id = {467034}, interhash = {f05a3fc91c66ef9782fc6b60d625e782}, intrahash = {ca1d616e2aedce2b7ac619764dd6fc50}, issn = {0009-9147}, journal = {Clin Chem}, keywords = {l-i-l-a automated complement immunoassay homogeneous ag_detection}, month = {June}, number = 6, pages = {808--812}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Automated homogeneous liposome immunoassay systems for anticonvulsant drugs.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=1597006}, volume = 38, year = 1992 }