%0 Journal Article %1 ijbiotech %A Wijayanti, Nastiti %A Wibawa, Tri %A Nirwati, Hera %A Haryanto, Aris %A ., Sutaryo %D 2006 %I Research Center for Biotechnology %J Indonesian Journal of Biotechnology %K Flavivirus, PCR RT-PCR, dengue multiplex nested serotype, virus, %N 2 %P 928-932 %T Rapid Detection and Molecular Typing of Dengue Virus by Using Multiplex-Nested-RT-PCR %U http://ijbiotech.ugm.ac.id/index.php/biotech/article/view/85 %V 11 %X The dengue viruses (genus Flavivirus) are mosquito borne and cause dengue fever in most tropical areas of the world. We have evaluated the combination of one-step RT-PCR and multiplex nested PCR assays for detecting dengue viruses from clinical samples. Twelve patients were screened for the dengue virus, using a pair of primers that conserve for several Flavivirus. The results showed that in 12 suspect patients, 100% were positive for Flavivirus and there are some genotypic variation among them, that indicated by several RT-PCR products higher than 511 bp, the expected product for RT-PCR. Further assay was performed to clarify the presence and serotypes of dengue virus using multiplex nested PCR. Serotyping results indicated that 83,3% of samples can be confirmed for dengue virus. Among the dengue virus positive 16,7 % are dengue-2, 16.7 % are dengue-3, and the most common 50% are dengue-4, whereas dengue-1 were not found among the patients. The combination of RT-PCR and multiplex nested PCR assay can be used for rapid analysis dengue samples in early phase which is potentially useful for clinical, epidemiology and also evolutionary studies.

@article{ijbiotech, abstract = {The dengue viruses (genus Flavivirus) are mosquito borne and cause dengue fever in most tropical areas of the world. We have evaluated the combination of one-step RT-PCR and multiplex nested PCR assays for detecting dengue viruses from clinical samples. Twelve patients were screened for the dengue virus, using a pair of primers that conserve for several Flavivirus. The results showed that in 12 suspect patients, 100% were positive for Flavivirus and there are some genotypic variation among them, that indicated by several RT-PCR products higher than 511 bp, the expected product for RT-PCR. Further assay was performed to clarify the presence and serotypes of dengue virus using multiplex nested PCR. Serotyping results indicated that 83,3% of samples can be confirmed for dengue virus. Among the dengue virus positive 16,7 % are dengue-2, 16.7 % are dengue-3, and the most common 50% are dengue-4, whereas dengue-1 were not found among the patients. The combination of RT-PCR and multiplex nested PCR assay can be used for rapid analysis dengue samples in early phase which is potentially useful for clinical, epidemiology and also evolutionary studies.}, added-at = {2010-12-15T00:27:17.000+0100}, author = {Wijayanti, Nastiti and Wibawa, Tri and Nirwati, Hera and Haryanto, Aris and ., Sutaryo}, biburl = {https://www.bibsonomy.org/bibtex/2aea8a4d9db32bb03a0f6f38de2d37175/ijbiotech}, interhash = {f8d43e0d847af3aa8a427e152dc264b3}, intrahash = {aea8a4d9db32bb03a0f6f38de2d37175}, journal = {Indonesian Journal of Biotechnology}, keywords = {Flavivirus, PCR RT-PCR, dengue multiplex nested serotype, virus,}, number = 2, pages = {928-932}, publisher = {Research Center for Biotechnology}, timestamp = {2010-12-15T00:27:19.000+0100}, title = {Rapid Detection and Molecular Typing of Dengue Virus by Using Multiplex-Nested-RT-PCR}, url = {http://ijbiotech.ugm.ac.id/index.php/biotech/article/view/85}, volume = 11, year = 2006 }