%0 Journal Article %1 Ternes2002 %A Ternes, Thomas A. %A Andersen, Henrik %A Gilberg, Daniel %A Bonerz, Matthias %D 2002 %J Analytical Chemistry %K 6410 %N 14 %P 3498-3504 %R 10.1021/ac015717z %T Determination of Estrogens in Sludge and Sediments by Liquid Extraction and GC/MS/MS %U http://pubs.acs.org/doi/abs/10.1021/ac015717z %V 74 %X Two methods have been developed that enable the determination of estrogens down to 2 ng/g in digested and activated sludge from domestic sewage treatment plants (STPs) and down to 0.2 ng/g in freshwater sediments. The method for sludge analysis consists of solvent extraction; a gel permeation chromatography (GPC) cleanup step, a 1 g silica gel column; and finally, detection by GC-ion trap MS/MS of the silylated estrogens with MSTFA. For sediments, the solvent extraction was successively followed by silica gel cleanup, solid phase enrichment (SPE), and a HPLC cleanup before derivatization and GC/MS/MS detection. Mean recoveries of the estrogens mainly exceeded 70% in sludge and 90% in sediments. In activated and digested sewage sludge, estrone and 17beta-estradiol were detected up to 37 ng/g and 49 ng/g, respectively, and 17alpha-ethinylestradiol up to 17 ng/g. The occurrence of estrogens in digested sludge indicates that estrogens can be persistent during sludge digestion. In river sediments, estrone and 17beta-estradiol were detected up to 2 ng/g (estrone), and the contraceptive 17alpha-ethinylestradiol was found with a maximum of 0.9 ng/g. Mestranol, a prodrug for 17alpha-ethinylestradiol, was not detected either in sludge or in sediments.

@article{Ternes2002, abstract = { Two methods have been developed that enable the determination of estrogens down to 2 ng/g in digested and activated sludge from domestic sewage treatment plants (STPs) and down to 0.2 ng/g in freshwater sediments. The method for sludge analysis consists of solvent extraction; a gel permeation chromatography (GPC) cleanup step, a 1 g silica gel column; and finally, detection by GC-ion trap MS/MS of the silylated estrogens with MSTFA. For sediments, the solvent extraction was successively followed by silica gel cleanup, solid phase enrichment (SPE), and a HPLC cleanup before derivatization and GC/MS/MS detection. Mean recoveries of the estrogens mainly exceeded 70% in sludge and 90% in sediments. In activated and digested sewage sludge, estrone and 17beta-estradiol were detected up to 37 ng/g and 49 ng/g, respectively, and 17alpha-ethinylestradiol up to 17 ng/g. The occurrence of estrogens in digested sludge indicates that estrogens can be persistent during sludge digestion. In river sediments, estrone and 17beta-estradiol were detected up to 2 ng/g (estrone), and the contraceptive 17alpha-ethinylestradiol was found with a maximum of 0.9 ng/g. Mestranol, a prodrug for 17alpha-ethinylestradiol, was not detected either in sludge or in sediments. }, added-at = {2011-10-01T01:22:29.000+0200}, author = {Ternes, Thomas A. and Andersen, Henrik and Gilberg, Daniel and Bonerz, Matthias}, biburl = {https://www.bibsonomy.org/bibtex/2ac50e76a42412e66639ef42c652bea0f/afcallender}, doi = {10.1021/ac015717z}, eprint = {http://pubs.acs.org/doi/pdf/10.1021/ac015717z}, file = {Ternes2002.pdf:indexed\\Ternes2002.pdf:PDF}, groups = {public}, interhash = {c57a27624662eb2f778f35608e4680b1}, intrahash = {ac50e76a42412e66639ef42c652bea0f}, journal = {Analytical Chemistry}, keywords = {6410}, number = 14, pages = {3498-3504}, timestamp = {2011-10-01T01:22:29.000+0200}, title = {Determination of Estrogens in Sludge and Sediments by Liquid Extraction and GC/MS/MS}, url = {http://pubs.acs.org/doi/abs/10.1021/ac015717z}, username = {afcallender}, volume = 74, year = 2002 }