Recently, it has become possible to record the localized fluorescence
transient associated with the opening of a single plasma membrane
Ca$^2+$ permeable ion channel using Ca$^2+$ indicators like
fluo-3. These Single Channel Ca$^2+$ Fluorescence Transients
(SCCaFTs) share some of the characteristics of such elementary events
as Ca$^2+$ sparks and Ca$^2+$ puffs caused by Ca$^2+$
release from intracellular stores (due to the opening of ryanodine
receptors and IP(3) receptors, respectively). In contrast to intracellular
Ca$^2+$ release events, SCCaFTs can be observed while simultaneously
recording the unitary channel currents using patch-clamp techniques
to verify the channel openings. Imaging SCCaFTs provides a way to
examine localized Ca$^2+$ handling in the vicinity of a channel
with a known Ca$^2+$ influx, to obtain the Ca$^2+$ current
passing through plasma membrane cation channels in near physiological
solutions, to localize Ca$^2+$ permeable ion channels on the
plasma membrane, and to estimate the Ca$^2+$ currents underlying
those elementary events where the Ca$^2+$ currents cannot be
recorded. Here we review studies of these fluorescence transients
associated with caffeine-activated channels, L-type Ca$^2+$ channels,
and stretch-activated channels. For the L-type Ca$^2+$ channel,
SCCaFTs have been termed sparklets. In addition, we discuss how SCCaFTs
have been used to estimate Ca$^2+$ currents using the rate of
rise of the fluorescence transient as well as the signal mass associated
with the total fluorescence increase.
%0 Journal Article
%1 Zou_2004_523
%A Zou, Hui
%A Lifshitz, Lawrence M
%A Tuft, Richard A
%A Fogarty, Kevin E
%A Singer, Joshua J
%D 2004
%J Cell Calcium
%K 15110142 Aniline Animals, Bufo Caffeine, Calcium Calcium, Cell Channel Channels, Compounds, Conductivity, Cytosol, Dyes, Electric Fluorescence, Fluorescent Gating, Gov't, In Ion Membr, Microscopy, Muscle, Myocytes, P.H.S., Patch-Clamp Research Smooth Support, Techniques, U.S. Vitro, Xanthenes, ane, marinus,
%N 6
%P 523--533
%R 10.1016/j.ceca.2004.01.019
%T Imaging calcium entering the cytosol through a single opening of
plasma membrane ion channels: SCCaFTs--fundamental calcium events.
%U http://dx.doi.org/10.1016/j.ceca.2004.01.019
%V 35
%X Recently, it has become possible to record the localized fluorescence
transient associated with the opening of a single plasma membrane
Ca$^2+$ permeable ion channel using Ca$^2+$ indicators like
fluo-3. These Single Channel Ca$^2+$ Fluorescence Transients
(SCCaFTs) share some of the characteristics of such elementary events
as Ca$^2+$ sparks and Ca$^2+$ puffs caused by Ca$^2+$
release from intracellular stores (due to the opening of ryanodine
receptors and IP(3) receptors, respectively). In contrast to intracellular
Ca$^2+$ release events, SCCaFTs can be observed while simultaneously
recording the unitary channel currents using patch-clamp techniques
to verify the channel openings. Imaging SCCaFTs provides a way to
examine localized Ca$^2+$ handling in the vicinity of a channel
with a known Ca$^2+$ influx, to obtain the Ca$^2+$ current
passing through plasma membrane cation channels in near physiological
solutions, to localize Ca$^2+$ permeable ion channels on the
plasma membrane, and to estimate the Ca$^2+$ currents underlying
those elementary events where the Ca$^2+$ currents cannot be
recorded. Here we review studies of these fluorescence transients
associated with caffeine-activated channels, L-type Ca$^2+$ channels,
and stretch-activated channels. For the L-type Ca$^2+$ channel,
SCCaFTs have been termed sparklets. In addition, we discuss how SCCaFTs
have been used to estimate Ca$^2+$ currents using the rate of
rise of the fluorescence transient as well as the signal mass associated
with the total fluorescence increase.
@article{Zou_2004_523,
abstract = {Recently, it has become possible to record the localized fluorescence
transient associated with the opening of a single plasma membrane
{C}a$^{2+}$ permeable ion channel using {C}a$^{2+}$ indicators like
fluo-3. These Single Channel {C}a$^{2+}$ Fluorescence Transients
(SCCaFTs) share some of the characteristics of such elementary events
as {C}a$^{2+}$ sparks and {C}a$^{2+}$ puffs caused by {C}a$^{2+}$
release from intracellular stores (due to the opening of ryanodine
receptors and IP(3) receptors, respectively). In contrast to intracellular
{C}a$^{2+}$ release events, SCCaFTs can be observed while simultaneously
recording the unitary channel currents using patch-clamp techniques
to verify the channel openings. Imaging SCCaFTs provides a way to
examine localized {C}a$^{2+}$ handling in the vicinity of a channel
with a known {C}a$^{2+}$ influx, to obtain the {C}a$^{2+}$ current
passing through plasma membrane cation channels in near physiological
solutions, to localize {C}a$^{2+}$ permeable ion channels on the
plasma membrane, and to estimate the {C}a$^{2+}$ currents underlying
those elementary events where the {C}a$^{2+}$ currents cannot be
recorded. Here we review studies of these fluorescence transients
associated with caffeine-activated channels, L-type {C}a$^{2+}$ channels,
and stretch-activated channels. For the L-type {C}a$^{2+}$ channel,
SCCaFTs have been termed sparklets. In addition, we discuss how SCCaFTs
have been used to estimate {C}a$^{2+}$ currents using the rate of
rise of the fluorescence transient as well as the signal mass associated
with the total fluorescence increase.},
added-at = {2009-06-03T11:20:58.000+0200},
author = {Zou, Hui and Lifshitz, Lawrence M and Tuft, Richard A and Fogarty, Kevin E and Singer, Joshua J},
biburl = {https://www.bibsonomy.org/bibtex/2c9032438a398ad3ef5ace916df6d75a9/hake},
description = {The whole bibliography file I use.},
doi = {10.1016/j.ceca.2004.01.019},
file = {Zou_2004_523.pdf:Zou_2004_523.pdf:PDF},
interhash = {3b2aabddecd3e6dbd158a439231d23c7},
intrahash = {c9032438a398ad3ef5ace916df6d75a9},
journal = {Cell Calcium},
key = 261,
keywords = {15110142 Aniline Animals, Bufo Caffeine, Calcium Calcium, Cell Channel Channels, Compounds, Conductivity, Cytosol, Dyes, Electric Fluorescence, Fluorescent Gating, Gov't, In Ion Membr, Microscopy, Muscle, Myocytes, P.H.S., Patch-Clamp Research Smooth Support, Techniques, U.S. Vitro, Xanthenes, ane, marinus,},
month = Jun,
number = 6,
pages = {523--533},
pii = {S0143416004000387},
pmid = {15110142},
timestamp = {2009-06-03T11:21:39.000+0200},
title = {Imaging calcium entering the cytosol through a single opening of
plasma membrane ion channels: {SCC}a{FT}s--fundamental calcium events.},
url = {http://dx.doi.org/10.1016/j.ceca.2004.01.019},
volume = 35,
year = 2004
}