Different efficacy of various blocking reagents to eliminate interferences by human antimouse antibodies with a two-site immunoassay
J. Reinsberg. Clin.Biochem., 29 (2):
145-148(1996)
Abstract
OBJECTIVE: The efficacy of three reagents to eliminate interferences with the cancer antigen 125 (CA-125) assay Enzymun CA-125 II by human antimouse antibodies (HAMA) formed by patients after injection of the murine anti-CA-125 antibody OC125 is compared. METHODS: Apparent CA-125 concentrations of 14 serum samples obtained from 6 patients after multiple injections of 1 mg radiolabeled OC125 F(ab')2 fragments were measured with the Enzymun CA-125 II before and after preincubation, either with nonspecific mouse IgG, with the polymerized mouse IgG MAK-33, or with the commercially available HAMA-blocking reagent IIR. RESULTS: In all samples with HAMA concentrations ranging from 341 to 46900 microg/L, false-positive CA-125 values were measured with the Enzymun CA-125 II, which could be reduced by preincubation with the HAMA-blocking reagents. However, although after preincubation with 2 g/L IIR for all samples the CA-125 concentrations measured were reduced to values within the normal range
%0 Journal Article
%1 Reinsberg.1996
%A Reinsberg, J.
%D 1996
%J Clin.Biochem.
%K Analysis Animals Antibodies Antigen Blocking Blood CA-125 Chemical Evaluation False Heterophile Human Humans Immunoassay Indicators Mice Positive RANGE Reactions Reagents Studies Topic and as blood methods
%N 2
%P 145-148
%T Different efficacy of various blocking reagents to eliminate interferences by human antimouse antibodies with a two-site immunoassay
%U PM:8601323
%V 29
%X OBJECTIVE: The efficacy of three reagents to eliminate interferences with the cancer antigen 125 (CA-125) assay Enzymun CA-125 II by human antimouse antibodies (HAMA) formed by patients after injection of the murine anti-CA-125 antibody OC125 is compared. METHODS: Apparent CA-125 concentrations of 14 serum samples obtained from 6 patients after multiple injections of 1 mg radiolabeled OC125 F(ab')2 fragments were measured with the Enzymun CA-125 II before and after preincubation, either with nonspecific mouse IgG, with the polymerized mouse IgG MAK-33, or with the commercially available HAMA-blocking reagent IIR. RESULTS: In all samples with HAMA concentrations ranging from 341 to 46900 microg/L, false-positive CA-125 values were measured with the Enzymun CA-125 II, which could be reduced by preincubation with the HAMA-blocking reagents. However, although after preincubation with 2 g/L IIR for all samples the CA-125 concentrations measured were reduced to values within the normal range
@article{Reinsberg.1996,
abstract = {OBJECTIVE: The efficacy of three reagents to eliminate interferences with the cancer antigen 125 (CA-125) assay Enzymun CA-125 II by human antimouse antibodies (HAMA) formed by patients after injection of the murine anti-CA-125 antibody OC125 is compared. METHODS: Apparent CA-125 concentrations of 14 serum samples obtained from 6 patients after multiple injections of 1 mg radiolabeled OC125 F(ab')2 fragments were measured with the Enzymun CA-125 II before and after preincubation, either with nonspecific mouse IgG, with the polymerized mouse IgG MAK-33, or with the commercially available HAMA-blocking reagent IIR. RESULTS: In all samples with HAMA concentrations ranging from 341 to 46900 microg/L, false-positive CA-125 values were measured with the Enzymun CA-125 II, which could be reduced by preincubation with the HAMA-blocking reagents. However, although after preincubation with 2 g/L IIR for all samples the CA-125 concentrations measured were reduced to values within the normal range},
added-at = {2010-02-05T11:28:39.000+0100},
author = {Reinsberg, J.},
biburl = {https://www.bibsonomy.org/bibtex/2e837b8fd00fd75946e4bd98c42da6828/kanefendt},
interhash = {cfe971fd7f8c056249359ef9caaf9677},
intrahash = {e837b8fd00fd75946e4bd98c42da6828},
journal = {Clin.Biochem.},
keywords = {Analysis Animals Antibodies Antigen Blocking Blood CA-125 Chemical Evaluation False Heterophile Human Humans Immunoassay Indicators Mice Positive RANGE Reactions Reagents Studies Topic and as blood methods},
number = 2,
pages = {145-148},
timestamp = {2010-02-05T11:28:44.000+0100},
title = {Different efficacy of various blocking reagents to eliminate interferences by human antimouse antibodies with a two-site immunoassay},
url = {PM:8601323},
volume = 29,
year = 1996
}