%0 Journal Article %1 Zimmermann2008a %A Zimmermann, D. %A Kiesel, M. %A Terpitz, U. %A Zhou, A. %A Reuss, R. %A Kraus, J. %A Schenk, W. A. %A Bamberg, E. %A Sukhorukov, V. L. %C 233 SPRING STREET, NEW YORK, NY 10013 USA %D 2008 %I SPRINGER %J JOURNAL OF MEMBRANE BIOLOGY %K terpitz vladimir %N 2 %P 107-121 %T A combined patch-clamp and electrorotation study of the voltage- and frequency-dependent membrane capacitance caused by structurally dissimilar lipophilic anions %U http://dx.doi.org/10.1007/s00232-007-9090-4 %V 221 %X Interactions of structurally dissimilar anionic compounds with the plasma membrane of HEK293 cells were analyzed by patch clamp and electrorotation. The combined approach provides complementary information on the lipophilicity, preferential affinity of the anions to the inner/outer membrane leaflet, adsorption depth and transmembrane mobility. The anionic species studied here included the well-known lipophilic anions dipicrylamine (DPA(-)), tetraphenylborate (TPB-) and W-2(CO)(10)(S2CH)(-), the putative lipophilic anion B(CF3)(4)(-) and three new heterocyclic W(CO)(5) derivatives. All tested anions partitioned strongly into the cell membrane, as indicated by the capacitance increase in patch-clamped cells. The capacitance increment exhibited a bell-shaped dependence on membrane voltage. The midpoint potentials of the maximum capacitance increment were negative, indicating the exclusion of lipophilic anions from the outer membrane leaflet. The adsorption depth of the large organic anions DPA(-), TPB- and B(CF3)(4)(-) increased and that of W(CO)(5) derivatives decreased with increasing concentration of mobile charges. In agreement with the patch-clamp data, electrorotation of cells treated with DPA(-) and W(CO)(5) derivatives revealed a large dispersion of membrane capacitance in the kilohertz to megahertz range due to the translocation of mobile charges. In contrast, in the presence of TPB- and B(CF3)(4)(-) no mobile charges could be detected by electrorotation, despite their strong membrane adsorption. Our data suggest that the presence of oxygen atoms in the outer molecular shell is an important factor for the fast translocation ability of lipophilic anions.

@article{Zimmermann2008a, abstract = {Interactions of structurally dissimilar anionic compounds with the plasma membrane of HEK293 cells were analyzed by patch clamp and electrorotation. The combined approach provides complementary information on the lipophilicity, preferential affinity of the anions to the inner/outer membrane leaflet, adsorption depth and transmembrane mobility. The anionic species studied here included the well-known lipophilic anions dipicrylamine (DPA(-)), tetraphenylborate (TPB-) and {[}W-2(CO)(10)(S2CH)](-), the putative lipophilic anion B(CF3)(4)(-) and three new heterocyclic W(CO)(5) derivatives. All tested anions partitioned strongly into the cell membrane, as indicated by the capacitance increase in patch-clamped cells. The capacitance increment exhibited a bell-shaped dependence on membrane voltage. The midpoint potentials of the maximum capacitance increment were negative, indicating the exclusion of lipophilic anions from the outer membrane leaflet. The adsorption depth of the large organic anions DPA(-), TPB- and B(CF3)(4)(-) increased and that of W(CO)(5) derivatives decreased with increasing concentration of mobile charges. In agreement with the patch-clamp data, electrorotation of cells treated with DPA(-) and W(CO)(5) derivatives revealed a large dispersion of membrane capacitance in the kilohertz to megahertz range due to the translocation of mobile charges. In contrast, in the presence of TPB- and B(CF3)(4)(-) no mobile charges could be detected by electrorotation, despite their strong membrane adsorption. Our data suggest that the presence of oxygen atoms in the outer molecular shell is an important factor for the fast translocation ability of lipophilic anions.}, added-at = {2011-03-02T09:35:06.000+0100}, address = {233 SPRING STREET, NEW YORK, NY 10013 USA}, affiliation = {Sukhorukov, VL (Reprint Author), Univ Wurzburg, Dept Biotechnol, D-97074 Wurzburg, Germany. {[}Kiesel, M.; Reuss, R.; Kraus, J.; Sukhorukov, V. L.] Univ Wurzburg, Dept Biotechnol, D-97074 Wurzburg, Germany. {[}Zimmermann, D.; Terpitz, U.; Zhou, A.; Kraus, J.; Bamberg, E.] Max Planck Inst Biophys, Dept Biophys Chem, D-60438 Frankfurt, Germany. {[}Zimmermann, D.; Bamberg, E.] Univ Frankfurt, Dept Biophys Chem Chem \& Pharm, D-60438 Frankfurt, Germany. {[}Schenk, W. A.] Univ Wurzburg, Inst Inorgan Chem, D-97074 Wurzburg, Germany.}, author = {Zimmermann, D. and Kiesel, M. and Terpitz, U. and Zhou, A. and Reuss, R. and Kraus, J. and Schenk, W. A. and Bamberg, E. and Sukhorukov, V. L.}, author-email = {sukhorukov@biozentrum.uni-wuerzburg.de}, biburl = {https://www.bibsonomy.org/bibtex/2d9fe379ccba0973eeffac62b6fcaa7ee/reichert}, doc-delivery-number = {259NA}, groups = {public}, interhash = {002baabd22b9b23d267b16f1049fa59d}, intrahash = {d9fe379ccba0973eeffac62b6fcaa7ee}, issn = {0022-2631}, journal = {JOURNAL OF MEMBRANE BIOLOGY}, journal-iso = {J. Membr. Biol.}, keywords = {terpitz vladimir}, keywords-plus = {LIPID BILAYER-MEMBRANES; CHARGE-PULSE RELAXATION; OUTER HAIR-CELLS; PLASMA-MEMBRANE; HYDROPHOBIC IONS; MAMMALIAN-CELLS; ELECTRO-ROTATION; GATING CURRENTS; TRANSPORT; DIPICRYLAMINE}, language = {English}, month = JAN, number = 2, number-of-cited-references = {51}, pages = {107-121}, publisher = {SPRINGER}, subject-category = {Biochemistry \& Molecular Biology; Cell Biology; Physiology}, times-cited = {6}, timestamp = {2011-03-09T14:16:50.000+0100}, title = {A combined patch-clamp and electrorotation study of the voltage- and frequency-dependent membrane capacitance caused by structurally dissimilar lipophilic anions}, type = {Article}, unique-id = {ISI:000252944800005}, url = {http://dx.doi.org/10.1007/s00232-007-9090-4}, username = {reichert}, volume = 221, year = 2008 }