%0 Journal Article %1 citeulike:780932 %A Radi, A. E. %A Sánchez, J. L. Acero %A Baldrich, E. %A O'Sullivan, C. K. %C Department of Chemistry, Faculty of Science, Mansoura University, 34517 Dumyat, Egypt. abd.radi@urv.net %D 2005 %J Anal Chem %K aptamer surface electrochemistry %N 19 %P 6320--6323 %R 10.1021/ac0505775 %T Reusable impedimetric aptasensor. %U http://dx.doi.org/10.1021/ac0505775 %V 77 %X A novel impedimetric aptasensor using a mixed self-assembled monolayer composed of thiol-modified thrombin binding aptamer and 2-mercaptoethanol on a gold electrode is reported. The changes of interfacial features of the electrode were probed in the presence of the reversible redox couple, Fe(CN)6(3-/4-), using impedance measurements. The electrode surface was partially blocked due to the self-assembly of aptamer or the formation of the aptamer-thrombin complex, resulting in an increase of the interfacial electron-transfer resistance detected by electrochemical impedance spectroscopy or cyclic voltammetry. The aptasensor was regenerated by breaking the complex formed between the aptamer and thrombin using 2.0 M NaCl solution, and the immobilized aptamer subsequently was used for repeated detection of thrombin. The aptamer-functionalized electrode showed a linear response of the charge-transfer resistance to the increase of thrombin concentration in the range of 5.0-35.0 nM and the thrombin was easily detectable to a concentration of 2.0 nM.

@article{citeulike:780932, abstract = {A novel impedimetric aptasensor using a mixed self-assembled monolayer composed of thiol-modified thrombin binding aptamer and 2-mercaptoethanol on a gold electrode is reported. The changes of interfacial features of the electrode were probed in the presence of the reversible redox couple, Fe(CN)6(3-/4-), using impedance measurements. The electrode surface was partially blocked due to the self-assembly of aptamer or the formation of the aptamer-thrombin complex, resulting in an increase of the interfacial electron-transfer resistance detected by electrochemical impedance spectroscopy or cyclic voltammetry. The aptasensor was regenerated by breaking the complex formed between the aptamer and thrombin using 2.0 M NaCl solution, and the immobilized aptamer subsequently was used for repeated detection of thrombin. The aptamer-functionalized electrode showed a linear response of the charge-transfer resistance to the increase of thrombin concentration in the range of 5.0-35.0 nM and the thrombin was easily detectable to a concentration of 2.0 nM.}, added-at = {2006-07-31T20:01:51.000+0200}, address = {Department of Chemistry, Faculty of Science, Mansoura University, 34517 Dumyat, Egypt. abd.radi@urv.net}, author = {Radi, A. E. and Sánchez, J. L. Acero and Baldrich, E. and O'Sullivan, C. K.}, biburl = {https://www.bibsonomy.org/bibtex/2670c551b29a95d6bf1dcd1b98d85c452/biblio24}, citeulike-article-id = {780932}, doi = {10.1021/ac0505775}, interhash = {026bf667057f1614bf7bceeede717cdb}, intrahash = {670c551b29a95d6bf1dcd1b98d85c452}, issn = {0003-2700}, journal = {Anal Chem}, keywords = {aptamer surface electrochemistry}, month = {October}, number = 19, pages = {6320--6323}, priority = {2}, timestamp = {2006-07-31T20:01:51.000+0200}, title = {Reusable impedimetric aptasensor.}, url = {http://dx.doi.org/10.1021/ac0505775}, volume = 77, year = 2005 }