%0 Journal Article %1 https://doi.org/10.1111/jnc.15346 %A Habenstein, Jens %A Schmitt, Franziska %A Liessem, Sander %A Ly, Alice %A Trede, Dennis %A Wegener, Christian %A Predel, Reinhard %A Rössler, Wolfgang %A Neupert, Susanne %D 2021 %J Journal of Neurochemistry %K ag_roessler zoo_2 %P 391–412 %R https://doi.org/10.1111/jnc.15346 %T Transcriptomic, peptidomic and mass spectrometry imaging analysis of the brain in the ant <i>Cataglyphis nodus</i> %U https://onlinelibrary.wiley.com/doi/abs/10.1111/jnc.15346 %V 158 %X Abstract Behavioral flexibility is an important cornerstone for the ecological success of animals. Social Cataglyphis nodus ants with their age-related polyethism characterized by age-related behavioral phenotypes represent a prime example for behavioral flexibility. We propose neuropeptides as powerful candidates for the flexible modulation of age-related behavioral transitions in individual ants. As the neuropeptidome of C. nodus was unknown, we collected a comprehensive peptidomic data set obtained by transcriptome analysis of the ants’ central nervous system combined with brain extract analysis by Q-Exactive Orbitrap mass spectrometry (MS) and direct tissue profiling of different regions of the brain by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS. In total, we identified 71 peptides with likely bioactive function, encoded on 49 neuropeptide-, neuropeptide-like and protein hormone prepropeptide genes, including a novel neuropeptide-like gene (fliktin). We next characterized the spatial distribution of a subset of peptides encoded on 16 precursor proteins with high resolution by MALDI MS imaging (MALDI MSI) on 14 µm brain sections. The accuracy of our MSI data was confirmed by matching the immunostaining patterns for tachykinins with MSI ion images from consecutive brain sections. Our data provide a solid framework for future research into spatially resolved qualitative and quantitative peptidomic changes associated with stage-specific behavioral transitions and the functional role of neuropeptides in Cataglyphis ants.

@article{https://doi.org/10.1111/jnc.15346, abstract = {Abstract Behavioral flexibility is an important cornerstone for the ecological success of animals. Social Cataglyphis nodus ants with their age-related polyethism characterized by age-related behavioral phenotypes represent a prime example for behavioral flexibility. We propose neuropeptides as powerful candidates for the flexible modulation of age-related behavioral transitions in individual ants. As the neuropeptidome of C. nodus was unknown, we collected a comprehensive peptidomic data set obtained by transcriptome analysis of the ants’ central nervous system combined with brain extract analysis by Q-Exactive Orbitrap mass spectrometry (MS) and direct tissue profiling of different regions of the brain by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS. In total, we identified 71 peptides with likely bioactive function, encoded on 49 neuropeptide-, neuropeptide-like and protein hormone prepropeptide genes, including a novel neuropeptide-like gene (fliktin). We next characterized the spatial distribution of a subset of peptides encoded on 16 precursor proteins with high resolution by MALDI MS imaging (MALDI MSI) on 14 µm brain sections. The accuracy of our MSI data was confirmed by matching the immunostaining patterns for tachykinins with MSI ion images from consecutive brain sections. Our data provide a solid framework for future research into spatially resolved qualitative and quantitative peptidomic changes associated with stage-specific behavioral transitions and the functional role of neuropeptides in Cataglyphis ants.}, added-at = {2021-03-11T16:11:16.000+0100}, author = {Habenstein, Jens and Schmitt, Franziska and Liessem, Sander and Ly, Alice and Trede, Dennis and Wegener, Christian and Predel, Reinhard and Rössler, Wolfgang and Neupert, Susanne}, biburl = {https://www.bibsonomy.org/bibtex/295827446c695d09f10d48f153926d95d/zoologieii}, doi = {https://doi.org/10.1111/jnc.15346}, eprint = {https://onlinelibrary.wiley.com/doi/pdf/10.1111/jnc.15346}, interhash = {0da2069531a5183733520833dccb4c45}, intrahash = {95827446c695d09f10d48f153926d95d}, journal = {Journal of Neurochemistry}, keywords = {ag_roessler zoo_2}, pages = {391–412}, timestamp = {2021-07-18T15:05:09.000+0200}, title = {Transcriptomic, peptidomic and mass spectrometry imaging analysis of the brain in the ant <i>Cataglyphis nodus</i>}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/jnc.15346}, volume = 158, year = 2021 }