%0 Journal Article %1 li2010sequence %A Li, Ruiqiang %A Fan, Wei %A Tian, Geng %A Zhu, Hongmei %A He, Lin %A Cai, Jing %A Huang, Quanfei %A Cai, Qingle %A Li, Bo %A Bai, Yinqi %A Zhang, Zhihe %A Zhang, Yaping %A Wang, Wen %A Li, Jun %A Wei, Fuwen %A Li, Heng %A Jian, Min %A Li, Jianwen %A Zhang, Zhaolei %A Nielsen, Rasmus %A Li, Dawei %A Gu, Wanjun %A Yang, Zhentao %A Xuan, Zhaoling %A Ryder, Oliver A. %A Leung, Frederick Chi-Ching %A Zhou, Yan %A Cao, Jianjun %A Sun, Xiao %A Fu, Yonggui %A Fang, Xiaodong %A Guo, Xiaosen %A Wang, Bo %A Hou, Rong %A Shen, Fujun %A Mu, Bo %A Ni, Peixiang %A Lin, Runmao %A Qian, Wubin %A Wang, Guodong %A Yu, Chang %A Nie, Wenhui %A Wang, Jinhuan %A Wu, Zhigang %A Liang, Huiqing %A Min, Jiumeng %A Wu, Qi %A Cheng, Shifeng %A Ruan, Jue %A Wang, Mingwei %A Shi, Zhongbin %A Wen, Ming %A Liu, Binghang %A Ren, Xiaoli %A Zheng, Huisong %A Dong, Dong %A Cook, Kathleen %A Shan, Gao %A Zhang, Hao %A Kosiol, Carolin %A Xie, Xueying %A Lu, Zuhong %A Zheng, Hancheng %A Li, Yingrui %A Steiner, Cynthia C. %A Lam, Tommy Tsan-Yuk %A Lin, Siyuan %A Zhang, Qinghui %A Li, Guoqing %A Tian, Jing %A Gong, Timing %A Liu, Hongde %A Zhang, Dejin %A Fang, Lin %A Ye, Chen %A Zhang, Juanbin %A Hu, Wenbo %A Xu, Anlong %A Ren, Yuanyuan %A Zhang, Guojie %A Bruford, Michael W. %A Li, Qibin %A Ma, Lijia %A Guo, Yiran %A An, Na %A Hu, Yujie %A Zheng, Yang %A Shi, Yongyong %A Li, Zhiqiang %A Liu, Qing %A Chen, Yanling %A Zhao, Jing %A Qu, Ning %A Zhao, Shancen %A Tian, Feng %A Wang, Xiaoling %A Wang, Haiyin %A Xu, Lizhi %A Liu, Xiao %A Vinar, Tomas %A Wang, Yajun %A Lam, Tak-Wah %A Yiu, Siu-Ming %A Liu, Shiping %A Zhang, Hemin %A Li, Desheng %A Huang, Yan %A Wang, Xia %A Yang, Guohua %A Jiang, Zhi %A Wang, Junyi %A Qin, Nan %A Li, Li %A Li, Jingxiang %A Bolund, Lars %A Kristiansen, Karsten %A Wong, Gane Ka-Shu %A Olson, Maynard %A Zhang, Xiuqing %A Li, Songgang %A Yang, Huanming %A Wang, Jian %A Wang, Jun %D 2010 %I Macmillan Publishers Limited. All rights reserved %J Nature %K giant.panda k-mer panda %N 7279 %P 311--317 %R 10.1038/nature08696 %T The sequence and de novo assembly of the giant panda genome %V 463 %X Using next-generation sequencing technology alone, we have successfully generated and assembled a draft sequence of the giant panda genome. The assembled contigs (2.25 gigabases (Gb)) cover approximately 94% of the whole genome, and the remaining gaps (0.05 Gb) seem to contain carnivore-specific repeats and tandem repeats. Comparisons with the dog and human showed that the panda genome has a lower divergence rate. The assessment of panda genes potentially underlying some of its unique traits indicated that its bamboo diet might be more dependent on its gut microbiome than its own genetic composition. We also identified more than 2.7 million heterozygous single nucleotide polymorphisms in the diploid genome. Our data and analyses provide a foundation for promoting mammalian genetic research, and demonstrate the feasibility for using next-generation sequencing technologies for accurate, cost-effective and rapid de novo assembly of large eukaryotic genomes.

@article{li2010sequence, abstract = {Using next-generation sequencing technology alone, we have successfully generated and assembled a draft sequence of the giant panda genome. The assembled contigs (2.25 gigabases (Gb)) cover approximately 94% of the whole genome, and the remaining gaps (0.05 Gb) seem to contain carnivore-specific repeats and tandem repeats. Comparisons with the dog and human showed that the panda genome has a lower divergence rate. The assessment of panda genes potentially underlying some of its unique traits indicated that its bamboo diet might be more dependent on its gut microbiome than its own genetic composition. We also identified more than 2.7 million heterozygous single nucleotide polymorphisms in the diploid genome. Our data and analyses provide a foundation for promoting mammalian genetic research, and demonstrate the feasibility for using next-generation sequencing technologies for accurate, cost-effective and rapid de novo assembly of large eukaryotic genomes.}, added-at = {2013-01-30T19:42:31.000+0100}, author = {Li, Ruiqiang and Fan, Wei and Tian, Geng and Zhu, Hongmei and He, Lin and Cai, Jing and Huang, Quanfei and Cai, Qingle and Li, Bo and Bai, Yinqi and Zhang, Zhihe and Zhang, Yaping and Wang, Wen and Li, Jun and Wei, Fuwen and Li, Heng and Jian, Min and Li, Jianwen and Zhang, Zhaolei and Nielsen, Rasmus and Li, Dawei and Gu, Wanjun and Yang, Zhentao and Xuan, Zhaoling and Ryder, Oliver A. and Leung, Frederick Chi-Ching and Zhou, Yan and Cao, Jianjun and Sun, Xiao and Fu, Yonggui and Fang, Xiaodong and Guo, Xiaosen and Wang, Bo and Hou, Rong and Shen, Fujun and Mu, Bo and Ni, Peixiang and Lin, Runmao and Qian, Wubin and Wang, Guodong and Yu, Chang and Nie, Wenhui and Wang, Jinhuan and Wu, Zhigang and Liang, Huiqing and Min, Jiumeng and Wu, Qi and Cheng, Shifeng and Ruan, Jue and Wang, Mingwei and Shi, Zhongbin and Wen, Ming and Liu, Binghang and Ren, Xiaoli and Zheng, Huisong and Dong, Dong and Cook, Kathleen and Shan, Gao and Zhang, Hao and Kosiol, Carolin and Xie, Xueying and Lu, Zuhong and Zheng, Hancheng and Li, Yingrui and Steiner, Cynthia C. and Lam, Tommy Tsan-Yuk and Lin, Siyuan and Zhang, Qinghui and Li, Guoqing and Tian, Jing and Gong, Timing and Liu, Hongde and Zhang, Dejin and Fang, Lin and Ye, Chen and Zhang, Juanbin and Hu, Wenbo and Xu, Anlong and Ren, Yuanyuan and Zhang, Guojie and Bruford, Michael W. and Li, Qibin and Ma, Lijia and Guo, Yiran and An, Na and Hu, Yujie and Zheng, Yang and Shi, Yongyong and Li, Zhiqiang and Liu, Qing and Chen, Yanling and Zhao, Jing and Qu, Ning and Zhao, Shancen and Tian, Feng and Wang, Xiaoling and Wang, Haiyin and Xu, Lizhi and Liu, Xiao and Vinar, Tomas and Wang, Yajun and Lam, Tak-Wah and Yiu, Siu-Ming and Liu, Shiping and Zhang, Hemin and Li, Desheng and Huang, Yan and Wang, Xia and Yang, Guohua and Jiang, Zhi and Wang, Junyi and Qin, Nan and Li, Li and Li, Jingxiang and Bolund, Lars and Kristiansen, Karsten and Wong, Gane Ka-Shu and Olson, Maynard and Zhang, Xiuqing and Li, Songgang and Yang, Huanming and Wang, Jian and Wang, Jun}, biburl = {https://www.bibsonomy.org/bibtex/2f2533c170c1e5fc9c576cb7c19109003/ytyoun}, doi = {10.1038/nature08696}, interhash = {11bcecddc767b71bac915429be7a265c}, intrahash = {f2533c170c1e5fc9c576cb7c19109003}, issn = {00280836}, journal = {Nature}, keywords = {giant.panda k-mer panda}, month = jan, number = 7279, pages = {311--317}, publisher = {Macmillan Publishers Limited. All rights reserved}, timestamp = {2013-01-30T19:42:31.000+0100}, title = {The sequence and de novo assembly of the giant panda genome}, volume = 463, year = 2010 }