%0 Journal Article %1 Qin2012a %A Qin, Gang %A Liu, Yinping %A Zheng, Jian %A Xiang, Zheng %A Ng, Iris H Y. %A Malik Peiris, J. S. %A Lau, Yu-Lung %A Tu, Wenwei %D 2012 %J J Infect Dis %K facs mucosal pneumonia tlymphocytes virus %N 11 %P 1646--1653 %R 10.1093/infdis/jis253 %T Phenotypic and Functional Characterization of Human γδ T-Cell Subsets in Response to Influenza A Viruses. %U http://dx.doi.org/10.1093/infdis/jis253 %V 205 %X Like αβ T cells, human γδ T cells also have different subsets with distinct characteristics. Whether human Vγ9Vδ2 T cells have functionally different subsets in response to influenza A (fluA) viruses remains unknown. In this study, we show for the first time that both central (CD45RA(-)CD27(+)) and effector (CD45RA(-)CD27(-)) memory Vγ9Vδ2 T cells have similar levels of immediate interferon (IFN) γ and cytotoxic responses to human and avian fluA virus-infected cells. In contrast, CD56(+) Vγ9Vδ2 T cells have significantly higher cytotoxicity against fluA virus-infected cells compared with their CD56(-) counterparts, whereas both subsets have similar IFN-γ responses. We further demonstrate that the CD16-dependent degranulation pathway, but not antibody-dependent cell-mediated cytotoxicity, contribute to the superior cytotoxicity of CD56(+) Vγ9Vδ2 T cells. Our study provides further evidence for the phenotypic and functional characterization of human Vγ9Vδ2 T-cell subsets during fluA virus infection and may help improve the γδ T-cell-based immunotherapy for viral infection.

@article{Qin2012a, abstract = {Like αβ T cells, human γδ T cells also have different subsets with distinct characteristics. Whether human Vγ9Vδ2 T cells have functionally different subsets in response to influenza A (fluA) viruses remains unknown. In this study, we show for the first time that both central (CD45RA(-)CD27(+)) and effector (CD45RA(-)CD27(-)) memory Vγ9Vδ2 T cells have similar levels of immediate interferon (IFN) γ and cytotoxic responses to human and avian fluA virus-infected cells. In contrast, CD56(+) Vγ9Vδ2 T cells have significantly higher cytotoxicity against fluA virus-infected cells compared with their CD56(-) counterparts, whereas both subsets have similar IFN-γ responses. We further demonstrate that the CD16-dependent degranulation pathway, but not antibody-dependent cell-mediated cytotoxicity, contribute to the superior cytotoxicity of CD56(+) Vγ9Vδ2 T cells. Our study provides further evidence for the phenotypic and functional characterization of human Vγ9Vδ2 T-cell subsets during fluA virus infection and may help improve the γδ T-cell-based immunotherapy for viral infection.}, added-at = {2012-05-18T02:20:16.000+0200}, author = {Qin, Gang and Liu, Yinping and Zheng, Jian and Xiang, Zheng and Ng, Iris H Y. and {Malik Peiris}, J. S. and Lau, Yu-Lung and Tu, Wenwei}, biburl = {https://www.bibsonomy.org/bibtex/2a27ad866c6b2fc49f71eef90723dd8c4/aorchid}, doi = {10.1093/infdis/jis253}, file = {:allorejection/nkt/JInfectDis.205.1646.pdf:PDF}, groups = {public}, institution = {Departments of Paediatrics and Adolescent Medicine.}, interhash = {1289f976d2d69e3ea9646ef68cc02574}, intrahash = {a27ad866c6b2fc49f71eef90723dd8c4}, journal = {J Infect Dis}, keywords = {facs mucosal pneumonia tlymphocytes virus}, language = {eng}, medline-pst = {ppublish}, month = Jun, number = 11, pages = {1646--1653}, pii = {jis253}, pmid = {22457284}, timestamp = {2012-05-18T02:20:16.000+0200}, title = {Phenotypic and Functional Characterization of Human γδ T-Cell Subsets in Response to Influenza A Viruses.}, url = {http://dx.doi.org/10.1093/infdis/jis253}, username = {aorchid}, volume = 205, year = 2012 }