Article,
Marker-assisted increase of genetic diversity in a double-low seed quality winter oilseed rape genetic background
PLANT BREEDING, 126 (6): 581-587 (2007)
Abstract
Generation of novel genetic diversity for maximization of heterosis in
hybrid production is a significant goal in winter oilseed rape
breeding. Here, we demonstrate that doubled haploid (DH) production
using microspore cultivation can simultaneously introgress favourable
alleles for double-low seed quality (low erucic acid and
low-glucosinolate content) into a genetically diverse Brassica napus
genetic background. The DH lines were derived from a cross between a
double-low quality winter rapeseed variety and a genetically diverse
semisynthetic B. napus line with high erucic acid and high
glucosinolates (+ + quality). Twenty-three low-glucosinolate lines were
identified with a genome component of 50-67% derived from the + +
parent. Four of these lines, with a genome component of 50-55% derived
from the + + parent, also contained low erucic acid. Heterosis for seed
yield was confirmed in test-crosses using these genetically diverse
lines as pollinator. The results demonstrate the potential of
marker-assisted identification of novel genetic pools for breeding of
double-low quality winter oilseed rape hybrids.
