%0 Journal Article %1 Gwosch2020 %A Gwosch, Klaus C. %A Pape, Jasmin K. %A Balzarotti, Francisco %A Hoess, Philipp %A Ellenberg, Jan %A Ries, Jonas %A Hell, Stefan W. %D 2020 %J Nature Methods %K 3D imaging microscopy no_pdf superresolution %N 2 %R 10.1038/s41592-019-0688-0 %T MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells %V 17 %X The ultimate goal of biological super-resolution fluorescence microscopy is to provide three-dimensional resolution at the size scale of a fluorescent marker. Here we show that by localizing individual switchable fluorophores with a probing donut-shaped excitation beam, MINFLUX nanoscopy can provide resolutions in the range of 1 to 3 nm for structures in fixed and living cells. This progress has been facilitated by approaching each fluorophore iteratively with the probing-donut minimum, making the resolution essentially uniform and isotropic over scalable fields of view. MINFLUX imaging of nuclear pore complexes of a mammalian cell shows that this true nanometer-scale resolution is obtained in three dimensions and in two color channels. Relying on fewer detected photons than standard camera-based localization, MINFLUX nanoscopy is poised to open a new chapter in the imaging of protein complexes and distributions in fixed and living cells.

@article{Gwosch2020, abstract = {The ultimate goal of biological super-resolution fluorescence microscopy is to provide three-dimensional resolution at the size scale of a fluorescent marker. Here we show that by localizing individual switchable fluorophores with a probing donut-shaped excitation beam, MINFLUX nanoscopy can provide resolutions in the range of 1 to 3 nm for structures in fixed and living cells. This progress has been facilitated by approaching each fluorophore iteratively with the probing-donut minimum, making the resolution essentially uniform and isotropic over scalable fields of view. MINFLUX imaging of nuclear pore complexes of a mammalian cell shows that this true nanometer-scale resolution is obtained in three dimensions and in two color channels. Relying on fewer detected photons than standard camera-based localization, MINFLUX nanoscopy is poised to open a new chapter in the imaging of protein complexes and distributions in fixed and living cells.}, added-at = {2020-04-06T13:11:22.000+0200}, author = {Gwosch, Klaus C. and Pape, Jasmin K. and Balzarotti, Francisco and Hoess, Philipp and Ellenberg, Jan and Ries, Jonas and Hell, Stefan W.}, biburl = {https://www.bibsonomy.org/bibtex/2a84bae8ed78f515fd39e49ddf4666fd3/kfriedl}, doi = {10.1038/s41592-019-0688-0}, interhash = {18eaa2db06a74fee415653108c98a52d}, intrahash = {a84bae8ed78f515fd39e49ddf4666fd3}, issn = {15487105}, journal = {Nature Methods}, keywords = {3D imaging microscopy no_pdf superresolution}, number = 2, timestamp = {2020-04-06T14:36:31.000+0200}, title = {{MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells}}, volume = 17, year = 2020 }