%0 Journal Article %1 bournival_presence_2011 %A Bournival, Véronique %A Desjardins, Roxane %A Campbell, Shirley %A Roberge, Claude %A Doueik, Alexandre %A Gendron, Louis %A Payet, Marcel Daniel %A Gallo-Payet, Nicole %A Day, Robert %A Praud, Jean-Paul %D 2011 %J Experimental Lung Research %K imported %N 4 %P 205--211 %R 10.3109/01902148.2010.536610 %T Presence of task-1 channel in the laryngeal mucosa in the newborn lamb %U http://www.ncbi.nlm.nih.gov/pubmed/21309733 %V 37 %X Nearly 40 potassium channels have been described in respiratory epithelial cells. Of these are found several members of the 4-transmembrane domain, 2-pore K(+) channel family (K2P family), namely Twik-1 and -2, Trek-1 and -2, Task-2, -3, and -4, Thik-1, and KCNK7. The aim of this study was to verify whether the Twik-related acid-sensitive K(+) channel, subtype 1 (Task-1) (also known as KCNK3), is present in the laryngeal mucosa in the newborn lamb. Through the use of immunohistochemistry and nested polymerase chain reaction (PCR) amplification, results indicate that Task-1 protein and mRNA are present in the laryngeal mucosa, in both the ciliated, pseudostratified columnar (respiratory) epithelium and the nonkeratinized, stratified squamous epithelium. The complete ovine Task-1 protein sequence showed high homology levels with previously reported mouse, bovine, and human Task-1 sequences. This includes a complete homology for the C-terminal amino acid sequence, which is mandatory for protein trafficking to the cell membrane. These results represent the first demonstration that Task-1, a pH-sensitive channel responsible for setting membrane potential, is present in the laryngeal mucosa of a newborn mammal.

@article{bournival_presence_2011, abstract = {Nearly 40 potassium channels have been described in respiratory epithelial cells. Of these are found several members of the 4-transmembrane domain, 2-pore K(+) channel family {(K2P} family), namely Twik-1 and -2, Trek-1 and -2, Task-2, -3, and -4, Thik-1, and {KCNK7.} The aim of this study was to verify whether the Twik-related acid-sensitive K(+) channel, subtype 1 {(Task-1)} (also known as {KCNK3)}, is present in the laryngeal mucosa in the newborn lamb. Through the use of immunohistochemistry and nested polymerase chain reaction {(PCR)} amplification, results indicate that Task-1 protein and {mRNA} are present in the laryngeal mucosa, in both the ciliated, pseudostratified columnar (respiratory) epithelium and the nonkeratinized, stratified squamous epithelium. The complete ovine Task-1 protein sequence showed high homology levels with previously reported mouse, bovine, and human Task-1 sequences. This includes a complete homology for the C-terminal amino acid sequence, which is mandatory for protein trafficking to the cell membrane. These results represent the first demonstration that Task-1, a {pH-sensitive} channel responsible for setting membrane potential, is present in the laryngeal mucosa of a newborn mammal.}, added-at = {2011-08-03T21:06:35.000+0200}, author = {Bournival, Véronique and Desjardins, Roxane and Campbell, Shirley and Roberge, Claude and Doueik, Alexandre and Gendron, Louis and Payet, Marcel Daniel and {Gallo-Payet}, Nicole and Day, Robert and Praud, {Jean-Paul}}, biburl = {https://www.bibsonomy.org/bibtex/2315381b735b150b6515322bfd2d16e5f/crc_chus}, doi = {10.3109/01902148.2010.536610}, interhash = {1ab2b92db3d91ee9f23771bb6405f709}, intrahash = {315381b735b150b6515322bfd2d16e5f}, issn = {1521-0499}, journal = {Experimental Lung Research}, keywords = {imported}, month = may, note = {{PMID:} 21309733}, number = 4, pages = {205--211}, timestamp = {2011-08-03T21:10:03.000+0200}, title = {Presence of task-1 channel in the laryngeal mucosa in the newborn lamb}, url = {http://www.ncbi.nlm.nih.gov/pubmed/21309733}, volume = 37, year = 2011 }