%0 Journal Article %1 Heidema2007 %A Heidema, Jojanneke %A Lukens, Michaël V. %A van Maren, Wendy W C. %A van Dijk, Mariska E A. %A Otten, Henny G. %A van Vught, Adrianus J. %A van der Werff, Desiree B M. %A van Gestel, Sjef J P. %A Semple, Malcolm G. %A Smyth, Rosalind L. %A Kimpen, Jan L L. %A van Bleek, Grada M. %D 2007 %J J Immunol %K balf facs tlymphocytes virus mucosal %N 12 %P 8410--8417 %T CD8+ T cell responses in bronchoalveolar lavage fluid and peripheral blood mononuclear cells of infants with severe primary respiratory syncytial virus infections. %U http://www.jimmunol.org/content/179/12/8410 %V 179 %X A protective role for CD8+ T cells during viral infections is generally accepted, but little is known about how CD8+ T cell responses develop during primary infections in infants, their efficacy, and how memory is established after viral clearance. We studied CD8+ T cell responses in bronchoalveolar lavage (BAL) samples and blood of infants with a severe primary respiratory syncytial virus (RSV) infection. RSV-specific CD8+ T cells with an activated effector cell phenotype: CD27+CD28+CD45RO+CCR7-CD38+HLA-DR+Granzyme B+CD127- could be identified in BAL and blood. A high proportion of these CD8+ T cells proliferated and functionally responded upon in vitro stimulation with RSV Ag. Thus, despite the very young age of the patients, a robust systemic virus-specific CD8+ T cell response was elicited against a localized respiratory infection. RSV-specific T cell numbers as well as the total number of activated effector type CD8+ T cells peaked in blood around day 9-12 after the onset of primary symptoms, i.e., at the time of recovery. The lack of a correlation between RSV-specific T cell numbers and parameters of disease severity make a prominent role in immune pathology unlikely, in contrast the T cells might be involved in the recovery process.

@article{Heidema2007, abstract = {A protective role for CD8+ T cells during viral infections is generally accepted, but little is known about how CD8+ T cell responses develop during primary infections in infants, their efficacy, and how memory is established after viral clearance. We studied CD8+ T cell responses in bronchoalveolar lavage (BAL) samples and blood of infants with a severe primary respiratory syncytial virus (RSV) infection. RSV-specific CD8+ T cells with an activated effector cell phenotype: CD27+CD28+CD45RO+CCR7-CD38+HLA-DR+Granzyme B+CD127- could be identified in BAL and blood. A high proportion of these CD8+ T cells proliferated and functionally responded upon in vitro stimulation with RSV Ag. Thus, despite the very young age of the patients, a robust systemic virus-specific CD8+ T cell response was elicited against a localized respiratory infection. RSV-specific T cell numbers as well as the total number of activated effector type CD8+ T cells peaked in blood around day 9-12 after the onset of primary symptoms, i.e., at the time of recovery. The lack of a correlation between RSV-specific T cell numbers and parameters of disease severity make a prominent role in immune pathology unlikely, in contrast the T cells might be involved in the recovery process.}, added-at = {2013-06-08T04:18:29.000+0200}, author = {Heidema, Jojanneke and Lukens, Michaël V. and {van Maren}, Wendy W C. and {van Dijk}, Mariska E A. and Otten, Henny G. and {van Vught}, Adrianus J. and {van der Werff}, Desiree B M. and {van Gestel}, Sjef J P. and Semple, Malcolm G. and Smyth, Rosalind L. and Kimpen, Jan L L. and {van Bleek}, Grada M.}, biburl = {https://www.bibsonomy.org/bibtex/2e2489c79865cd2d9c0dd0ee766121633/aorchid}, description = {see page 100 of notebook 00002. Unfortunately no BALF FACS pictures. Used calibur so no way measured all markers on same cell.}, file = {:allorejection/JImmunol.179.8410.pdf:PDF}, groups = {public}, institution = {Division of Pediatrics, Wilhelmina Children's Hospital, University Medical Center, Utrecht, The Netherlands.}, interhash = {1cc19bee23cf0a9b6fcb837ca18e1606}, intrahash = {e2489c79865cd2d9c0dd0ee766121633}, journal = {J Immunol}, keywords = {balf facs tlymphocytes virus mucosal}, language = {eng}, medline-pst = {ppublish}, month = Dec, number = 12, pages = {8410--8417}, pii = {179/12/8410}, pmid = {18056387}, timestamp = {2013-06-08T04:18:29.000+0200}, title = {CD8+ T cell responses in bronchoalveolar lavage fluid and peripheral blood mononuclear cells of infants with severe primary respiratory syncytial virus infections.}, url = {http://www.jimmunol.org/content/179/12/8410}, username = {aorchid}, volume = 179, year = 2007 }