%0 Journal Article %1 Asghary:2015-05-01T00:00:00:1533-4880:3394 %A Asghary, Maryam %A Raoof, Jahan-Bakhsh %A Hamidi-Asl, Ezat %A Ojani, Reza %D 2015-05-01T00:00:00 %J Journal of Nanoscience and Nanotechnology %K nanoscience %N 5 %P 3394-3404 %R 10.1166/jnn.2015.10211 %T A Novel Electrochemical Genosensor Based on Banana and Nano-Gold Modified Electrode Using Tyrosinase Enzyme as Indicator %U http://www.ingentaconnect.com/content/asp/jnn/2015/00000015/00000005/art00011 %V 15 %X The electrochemical behavior of the tyrosinase enzyme at the surface of two electrodes, carbon paste electrode (CPE) and nano-gold modified carbon paste electrode (NGCPE), has been studied by cyclic voltammetry. Tyrosinase showed one oxidation peak (around +0.85 V) and one reduction, peak at +0.40 V versus Ag|AgCl|KCl (3 M). To calculate the values of and ks, , the effect of potential scan rate on the electrochemical properties of tyrosinase was investigated. Cyclic voltammetry and UV-vis absorption techniques were used for the study of interaction, between DNA and tyrosinase. The cyclic voltammogram of tyrosinase was obtained in the presence of different types of DNA bases for the study of tyrosinase-DNA binding. The results showed that the hydrogen binding and electrostatic interactions were important interaction mode. Moreover, a variation, in tyrosinase signals intensity regarding the interaction to ssDNA and dsDNA was observed. The selectivity of the biosensor was studied using noncomplementary oligonucleotides. Finally, banana modified carbon paste electrode was also prepared to investigate the interaction of banana's tyrosinase, with DNA. The limit of detection for DNA probe was calculated 0.33 pM by using the oxidation signal of accumulated tyrosinase in NGCPE.

@article{Asghary:2015-05-01T00:00:00:1533-4880:3394, abstract = {The electrochemical behavior of the tyrosinase enzyme at the surface of two electrodes, carbon paste electrode (CPE) and nano-gold modified carbon paste electrode (NGCPE), has been studied by cyclic voltammetry. Tyrosinase showed one oxidation peak (around +0.85 V) and one reduction, peak at +0.40 V versus Ag|AgCl|KCl (3 M). To calculate the values of and ks, , the effect of potential scan rate on the electrochemical properties of tyrosinase was investigated. Cyclic voltammetry and UV-vis absorption techniques were used for the study of interaction, between DNA and tyrosinase. The cyclic voltammogram of tyrosinase was obtained in the presence of different types of DNA bases for the study of tyrosinase-DNA binding. The results showed that the hydrogen binding and electrostatic interactions were important interaction mode. Moreover, a variation, in tyrosinase signals intensity regarding the interaction to ssDNA and dsDNA was observed. The selectivity of the biosensor was studied using noncomplementary oligonucleotides. Finally, banana modified carbon paste electrode was also prepared to investigate the interaction of banana's tyrosinase, with DNA. The limit of detection for DNA probe was calculated 0.33 pM by using the oxidation signal of accumulated tyrosinase in NGCPE.}, added-at = {2014-10-07T14:21:20.000+0200}, author = {Asghary, Maryam and Raoof, Jahan-Bakhsh and Hamidi-Asl, Ezat and Ojani, Reza}, biburl = {https://www.bibsonomy.org/bibtex/2d1396e25e2b3036bd11ea1924109d123/liloubio}, description = {A Novel Electrochemical Genosensor Based on Banana and Nano-Gold ...: ingentaconnect}, doi = {10.1166/jnn.2015.10211}, interhash = {1ff26f82ebafa2a3719e40ed6738d761}, intrahash = {d1396e25e2b3036bd11ea1924109d123}, journal = {Journal of Nanoscience and Nanotechnology}, keywords = {nanoscience}, number = 5, pages = {3394-3404}, timestamp = {2014-10-07T14:21:20.000+0200}, title = {A Novel Electrochemical Genosensor Based on Banana and Nano-Gold Modified Electrode Using Tyrosinase Enzyme as Indicator}, url = {http://www.ingentaconnect.com/content/asp/jnn/2015/00000015/00000005/art00011}, volume = 15, year = {2015-05-01T00:00:00} }