%0 Journal Article %1 RN1201 %A Goh, C. H. %A Dietrich, P. %A Steinmeyer, R. %A Schreiber, U. %A Nam, H. G. %A Hedrich, R. %D 2002 %J Plant Journal %K atp myOwn %N 4 %P 623-630 %R DOI 10.1046/j.1365-313X.2002.01451.x %T Parallel recordings of photosynthetic electron transport and K -channel activity in single guard cells %U /brokenurl#<Go to ISI>://WOS:000179414100018 %V 32 %X Stomata open in response to red and blue light. Red light-induced stomatal movement is mediated by guard cell chloroplasts and related to K+-uptake into these motor cells. We have combined a new type of microchlorophyll fluorometer with the patch-clamp technique for parallel studies of the photosynthetic electron transport and activity of plasma membrane K+ channels in single guard cell protoplast. In the whole-cell configuration and presence of ATP in the patch-pipette, the activity of the K+-uptake channels remained constant throughout the course of an experiment (up to 30 min) while photosynthetic activity declined to about 50%. In the absence of ATP inward K+ currents declined in a time-dependent manner. Under these ATP-free conditions, photosynthetic electron transport was completely blocked within 8 min. ADP together with orthophosphate was able to prevent inhibition of photosynthetic electron transport and run-down of K+-channel activity. The results demonstrate that the combination of these two techniques is suited to directly study cytosolic factors as common regulators of photosynthesis and plasma membrane transport within a single-cell.

@article{RN1201, abstract = {Stomata open in response to red and blue light. Red light-induced stomatal movement is mediated by guard cell chloroplasts and related to K+-uptake into these motor cells. We have combined a new type of microchlorophyll fluorometer with the patch-clamp technique for parallel studies of the photosynthetic electron transport and activity of plasma membrane K+ channels in single guard cell protoplast. In the whole-cell configuration and presence of ATP in the patch-pipette, the activity of the K+-uptake channels remained constant throughout the course of an experiment (up to 30 min) while photosynthetic activity declined to about 50%. In the absence of ATP inward K+ currents declined in a time-dependent manner. Under these ATP-free conditions, photosynthetic electron transport was completely blocked within 8 min. ADP together with orthophosphate was able to prevent inhibition of photosynthetic electron transport and run-down of K+-channel activity. The results demonstrate that the combination of these two techniques is suited to directly study cytosolic factors as common regulators of photosynthesis and plasma membrane transport within a single-cell.}, added-at = {2024-02-14T14:38:32.000+0100}, author = {Goh, C. H. and Dietrich, P. and Steinmeyer, R. and Schreiber, U. and Nam, H. G. and Hedrich, R.}, biburl = {https://www.bibsonomy.org/bibtex/2d74d120df1b1443ed4fe0bb569b6c912/rainerhedrich_2}, doi = {DOI 10.1046/j.1365-313X.2002.01451.x}, interhash = {20b591344ebf9a541b614ad2388098ec}, intrahash = {d74d120df1b1443ed4fe0bb569b6c912}, issn = {0960-7412}, journal = {Plant Journal}, keywords = {atp myOwn}, note = {618jc Times Cited:23 Cited References Count:31}, number = 4, pages = {623-630}, timestamp = {2024-02-14T14:38:32.000+0100}, title = {Parallel recordings of photosynthetic electron transport and K -channel activity in single guard cells}, type = {Journal Article}, url = {/brokenurl#<Go to ISI>://WOS:000179414100018}, volume = 32, year = 2002 }