%0 Journal Article %1 Barton2014Integrated %A Barton, Nelson R. %A Burgard, Anthony P. %A Burk, Mark J. %A Crater, Jason S. %A Osterhout, Robin E. %A Pharkya, Priti %A Steer, Brian A. %A Sun, Jun %A Trawick, John D. %A Van Dien, Stephen J. %A Yang, Tae Hoon H. %A Yim, Harry %D 2014 %J Journal of industrial microbiology & biotechnology %K in-silico metabolic-engineering %R 10.1007/s10295-014-1541-1 %T An integrated biotechnology platform for developing sustainable chemical processes. %U http://dx.doi.org/10.1007/s10295-014-1541-1 %X Genomatica has established an integrated computational/experimental metabolic engineering platform to design, create, and optimize novel high performance organisms and bioprocesses. Here we present our platform and its use to develop E. coli strains for production of the industrial chemical 1,4-butanediol (BDO) from sugars. A series of examples are given to demonstrate how a rational approach to strain engineering, including carefully designed diagnostic experiments, provided critical insights about pathway bottlenecks, byproducts, expression balancing, and commercial robustness, leading to a superior BDO production strain and process.

@article{Barton2014Integrated, abstract = {Genomatica has established an integrated computational/experimental metabolic engineering platform to design, create, and optimize novel high performance organisms and bioprocesses. Here we present our platform and its use to develop E. coli strains for production of the industrial chemical 1,4-butanediol ({BDO}) from sugars. A series of examples are given to demonstrate how a rational approach to strain engineering, including carefully designed diagnostic experiments, provided critical insights about pathway bottlenecks, byproducts, expression balancing, and commercial robustness, leading to a superior {BDO} production strain and process.}, added-at = {2018-12-02T16:09:07.000+0100}, author = {Barton, Nelson R. and Burgard, Anthony P. and Burk, Mark J. and Crater, Jason S. and Osterhout, Robin E. and Pharkya, Priti and Steer, Brian A. and Sun, Jun and Trawick, John D. and Van Dien, Stephen J. and Yang, Tae Hoon H. and Yim, Harry}, biburl = {https://www.bibsonomy.org/bibtex/22efdd51f641a3af5c310637e3545a654/karthikraman}, citeulike-article-id = {13466893}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s10295-014-1541-1}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/25416472}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=25416472}, day = 22, doi = {10.1007/s10295-014-1541-1}, interhash = {2210ed44a0beb7ef6e157d870bb30337}, intrahash = {2efdd51f641a3af5c310637e3545a654}, issn = {1476-5535}, journal = {Journal of industrial microbiology \& biotechnology}, keywords = {in-silico metabolic-engineering}, month = nov, pmid = {25416472}, posted-at = {2014-12-22 06:52:50}, priority = {2}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {An integrated biotechnology platform for developing sustainable chemical processes.}, url = {http://dx.doi.org/10.1007/s10295-014-1541-1}, year = 2014 }