%0 Journal Article %1 Zhu2013Protein %A Zhu, Jian %A Larman, H. Benjamin %A Gao, Geng %A Somwar, Romel %A Zhang, Zijuan %A Laserson, Uri %A Ciccia, Alberto %A Pavlova, Natalya %A Church, George %A Zhang, Wei %A Kesari, Santosh %A Elledge, Stephen J. %D 2013 %I Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. %J Nature Biotechnology %K protein-protein-interactions %N 4 %P 331--334 %R 10.1038/nbt.2539 %T Protein interaction discovery using parallel analysis of translated ORFs (PLATO) %U http://dx.doi.org/10.1038/nbt.2539 %V 31 %X Identifying physical interactions between proteins and other molecules is a critical aspect of biological analysis. Here we describe PLATO, an in vitro method for mapping such interactions by affinity enrichment of a library of full-length open reading frames displayed on ribosomes, followed by massively parallel analysis using DNA sequencing. We demonstrate the broad utility of the method for human proteins by identifying known and previously unidentified interacting partners of LYN kinase, patient autoantibodies, and the small-molecules gefitinib and dasatinib.

@article{Zhu2013Protein, abstract = { Identifying physical interactions between proteins and other molecules is a critical aspect of biological analysis. Here we describe {PLATO}, an in vitro method for mapping such interactions by affinity enrichment of a library of full-length open reading frames displayed on ribosomes, followed by massively parallel analysis using {DNA} sequencing. We demonstrate the broad utility of the method for human proteins by identifying known and previously unidentified interacting partners of {LYN} kinase, patient autoantibodies, and the small-molecules gefitinib and dasatinib. }, added-at = {2018-12-02T16:09:07.000+0100}, author = {Zhu, Jian and Larman, H. Benjamin and Gao, Geng and Somwar, Romel and Zhang, Zijuan and Laserson, Uri and Ciccia, Alberto and Pavlova, Natalya and Church, George and Zhang, Wei and Kesari, Santosh and Elledge, Stephen J.}, biburl = {https://www.bibsonomy.org/bibtex/2001e5726119ce7cf8c19baeceb59c1e4/karthikraman}, citeulike-article-id = {12245439}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nbt.2539}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nbt.2539}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/23503679}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=23503679}, day = 17, doi = {10.1038/nbt.2539}, interhash = {22869df09e7de6be03d97aab5dd56b91}, intrahash = {001e5726119ce7cf8c19baeceb59c1e4}, issn = {1087-0156}, journal = {Nature Biotechnology}, keywords = {protein-protein-interactions}, month = mar, number = 4, pages = {331--334}, pmid = {23503679}, posted-at = {2013-06-14 05:41:51}, priority = {2}, publisher = {Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {Protein interaction discovery using parallel analysis of translated {ORFs} ({PLATO})}, url = {http://dx.doi.org/10.1038/nbt.2539}, volume = 31, year = 2013 }