%0 Journal Article %1 Geisenberger:2015:Acta-Neuropathol:25931051 %A Geisenberger, C %A Mock, A %A Warta, R %A Rapp, C %A Schwager, C %A Korshunov, A %A Nied, A K %A Capper, D %A Brors, B %A Jungk, C %A Jones, D %A Collins, V P %A Ichimura, K %A Bäcklund, L M %A Schnabel, E %A Mittelbron, M %A Lahrmann, B %A Zheng, S %A Verhaak, R G %A Grabe, N %A Pfister, S M %A Hartmann, C %A von Deimling, A %A Debus, J %A Unterberg, A %A Abdollahi, A %A Herold-Mende, C %D 2015 %J Acta Neuropathol %K ABI-DKFZ %R 10.1007/s00401-015-1427-y %T Molecular profiling of long-term survivors identifies a subgroup of glioblastoma characterized by chromosome 19/20 co-gain %U http://www.ncbi.nlm.nih.gov/pubmed/25931051 %X Glioblastoma (GBM) is a devastating tumor and few patients survive beyond 3�years. Defining the molecular determinants underlying long-term survival is essential for insights into tumor biology and biomarker identification. We therefore investigated homogeneously treated, IDH (wt) long-term (LTS, n�=�10) and short-term survivors (STS, n�=�6) by microarray transcription profiling. While there was no association of clinical parameters and molecular subtypes with long-term survival, STS tumors were characterized by differential polarization of infiltrating microglia with predominance of the M2 phenotype detectable both on the mRNA and protein level. Furthermore, transcriptional signatures of LTS and STS predicted patient outcome in a large, IDH (wt) cohort (n�=�468). Interrogation of overlapping genomic alterations identified concurrent gain of chromosomes 19 and 20 as a favorable prognostic marker. The strong association of this co-gain with survival was validated by aCGH in a second, independent cohort (n�=�124). Finally, FISH and gene expression data revealed gains to constitute low-amplitude, clonal events with a strong impact on transcription. In conclusion, these findings provide important insights into the manipulation of the innate immune system by particularly aggressive GBM tumors. Furthermore, we genomically characterize a previously unknown, clinically relevant subgroup of glioblastoma, which can easily be identified through modern neuropathological workup.

@article{Geisenberger:2015:Acta-Neuropathol:25931051, abstract = {Glioblastoma (GBM) is a devastating tumor and few patients survive beyond 3�years. Defining the molecular determinants underlying long-term survival is essential for insights into tumor biology and biomarker identification. We therefore investigated homogeneously treated, IDH (wt) long-term (LTS, n�=�10) and short-term survivors (STS, n�=�6) by microarray transcription profiling. While there was no association of clinical parameters and molecular subtypes with long-term survival, STS tumors were characterized by differential polarization of infiltrating microglia with predominance of the M2 phenotype detectable both on the mRNA and protein level. Furthermore, transcriptional signatures of LTS and STS predicted patient outcome in a large, IDH (wt) cohort (n�=�468). Interrogation of overlapping genomic alterations identified concurrent gain of chromosomes 19 and 20 as a favorable prognostic marker. The strong association of this co-gain with survival was validated by aCGH in a second, independent cohort (n�=�124). Finally, FISH and gene expression data revealed gains to constitute low-amplitude, clonal events with a strong impact on transcription. In conclusion, these findings provide important insights into the manipulation of the innate immune system by particularly aggressive GBM tumors. Furthermore, we genomically characterize a previously unknown, clinically relevant subgroup of glioblastoma, which can easily be identified through modern neuropathological workup.}, added-at = {2015-06-29T14:32:37.000+0200}, author = {Geisenberger, C and Mock, A and Warta, R and Rapp, C and Schwager, C and Korshunov, A and Nied, A K and Capper, D and Brors, B and Jungk, C and Jones, D and Collins, V P and Ichimura, K and B{\"a}cklund, L M and Schnabel, E and Mittelbron, M and Lahrmann, B and Zheng, S and Verhaak, R G and Grabe, N and Pfister, S M and Hartmann, C and von Deimling, A and Debus, J and Unterberg, A and Abdollahi, A and Herold-Mende, C}, biburl = {https://www.bibsonomy.org/bibtex/2334a209ef87564e3e10541b87dbb5a4e/juraeva}, doi = {10.1007/s00401-015-1427-y}, interhash = {25ce568f6a743aa0a09131afce7edbd9}, intrahash = {334a209ef87564e3e10541b87dbb5a4e}, journal = {Acta Neuropathol}, keywords = {ABI-DKFZ}, month = may, pmid = {25931051}, timestamp = {2015-06-29T14:34:20.000+0200}, title = {Molecular profiling of long-term survivors identifies a subgroup of glioblastoma characterized by chromosome 19/20 co-gain}, url = {http://www.ncbi.nlm.nih.gov/pubmed/25931051}, year = 2015 }