%0 Journal Article %1 sick2000orientational %A Sick, Beate %A Hecht, Bert %A Novotny, Lukas %D 2000 %J Phys. Rev. Lett. %K experiment nano-optics single-molecules %N 21 %P 4482-4485 %R 10.1103/PhysRevLett.85.4482 %T Orientational Imaging of Single Molecules by Annular Illumination %V 85 %X The absorption dipole orientation of single fluorescent molecules is determined by mapping the spatial distribution of the squared electric field components in a high-numerical-aperture laser focus. Annular illumination geometry and the vicinity of a plane dielectric/air interface strongly enhance the longitudinal field component and the transverse fields perpendicular to the polarization direction. As a result, all three excitation field components in the focus are of comparable magnitude. The scheme holds promise to monitor rotational diffusion of single molecules in complex environments.

@article{sick2000orientational, abstract = {The absorption dipole orientation of single fluorescent molecules is determined by mapping the spatial distribution of the squared electric field components in a high-numerical-aperture laser focus. Annular illumination geometry and the vicinity of a plane dielectric/air interface strongly enhance the longitudinal field component and the transverse fields perpendicular to the polarization direction. As a result, all three excitation field components in the focus are of comparable magnitude. The scheme holds promise to monitor rotational diffusion of single molecules in complex environments.}, added-at = {2020-02-10T14:56:53.000+0100}, author = {Sick, Beate and Hecht, Bert and Novotny, Lukas}, biburl = {https://www.bibsonomy.org/bibtex/251303769137085856c2526248a275d0d/ep5optics}, day = 20, doi = {10.1103/PhysRevLett.85.4482}, file = {APS Snapshot:C\:\\Users\\scherzad\\Zotero\\storage\\23VF4L8Z\\PhysRevLett.85.html:text/html;Sick et al. - 2000 - Orientational Imaging of Single Molecules by Annul.pdf:C\:\\Users\\scherzad\\Zotero\\storage\\RU879GFZ\\Sick et al. - 2000 - Orientational Imaging of Single Molecules by Annul.pdf:application/pdf}, interhash = {2a3632ad2086e10304e0f254c0e1b872}, intrahash = {51303769137085856c2526248a275d0d}, journal = {Phys. Rev. Lett.}, keywords = {experiment nano-optics single-molecules}, month = {11}, number = 21, pages = {4482-4485}, timestamp = {2020-02-10T14:56:53.000+0100}, title = {Orientational Imaging of Single Molecules by Annular Illumination}, urldate = {2020-02-10}, volume = 85, year = 2000 }